RNA processing. Part A, General methods /

Many methods have been developed or adapted for the study of RNA processing, which plays a key role in the expression of genetic information. Volumes 180 and 181 of Methods in Enzymology, RNA Processing, Parts A and B, represent the first comprehensive collation of protocols used for the preparation...

Πλήρης περιγραφή

Λεπτομέρειες βιβλιογραφικής εγγραφής
Άλλοι συγγραφείς: Dahlberg, James, Abelson, John
Μορφή: Ηλ. βιβλίο
Γλώσσα:English
Έκδοση: San Diego : Academic Press, ©1989.
Σειρά:Methods in enzymology ; v. 180.
Θέματα:
Διαθέσιμο Online:Full Text via HEAL-Link
Πίνακας περιεχομένων:
  • Isolation of RNA / Shelby L. Berger [and others]
  • Purification of RNA molecules gel techniques / Toshimichi Ikemura, James E. Dahlberg and John N. Abelson
  • Purifying RNA by column chromatography / N. Kyle Tanner, James E. Dahlberg and John N. Abelson
  • Synthesis of long, capped transcripts in Vitro by SP6 and T7 RNA polymerases / Joel K. Yisraeli [and others]
  • Synthesis of small RNAs using T7 RNA polymerase / John F. Milligan [and others]
  • In vitro synthesis of end-mature, intron-containing transfer RNAs / Vicente M. Reyes [and others]
  • Preparation of precursors to mRNA from mammalian cell nuclei / Selina Chen-Kiang [and others]
  • Pulse labeling of heterogeneous nuclear RNA in isolated nuclei / Selina Chen-Kiang [and others]
  • Isolation and characterization of yeast ribosomal RNA precursors and preribosomes / Jacobus Klootwuk [and others]
  • Preparation of yeast transfer RNA precursors in Vivo / Gayle Knapp, James E. Dahlberg and John N. Abelson
  • Dideoxy sequencing of RNA using reverse transcriptase / Chang Shahn [and others]
  • RNA fingerprinting / Andrea D. Branch [and others]
  • Enzymatic RNA sequencing / Y. Kuchino [and others]
  • Characterization of cap structures / Yasuhiro Furuichi [and others]
  • Isolation and characterization of branched oligonucleotides from RNA / J. David Reilly [and others]
  • Enzymatic approaches to probing of RNA secondary and tertiary structure / Gayle Knapp, James E. Dahlberg and John N. Abelson.
  • A guide for probing native small nuclear RNA and ribonucleoprotein structures / Alain Krol [and others]
  • Phylogenetic comparative analysis of RNA secondary structure / Bryan D. James [and others]
  • Mapping the genetic organization of RNA by electron microscopy / Louise T. Chow [and others]
  • Computer prediction of RNA structure / Michael Zuker, James E. Dahlberg and John N. Abelson
  • RNA pseudoknot: Structure, detection, and prediction / C.W.A. Pleij [and others]
  • absorbance melting curves of RNA / Joseph D. Puglisi [and others]
  • Nuclease digestion: A method for mapping introns / Shelby L. Berger, James E. Dahlberg and John N. Abelson
  • Characterization of RNA molecules by S1 nuclease analysis / Arnold J. Berk, James E. Dahlberg and John N. Abelson
  • Primer extension analysis of RNA / William R. Boorstein [and others]
  • Modification interference analysis of reactions using RNA substrates / Laura Conway [and others]
  • Photoaffinity cross-linking methods for studying RNA-protein interactions / Michelle M. Hanna, James E. Dahlberg and John N. Abelson
  • Ultraviolet-induced cross-linking of rna to proteins in vivo / Serafín Pinol-Roma [and others]
  • Analysis of ultraviolet-induced RNA-RNA cross-links: A means for probing RNA structure-function relationships / Andrea D. Branch [and others]
  • analysis of splicing complexes and small nuclear ribonucleoprotein particles by native gel electrophoresis / Maria M. Konarska, James E. Dahlberg and John N. Abelson
  • Determination of RNA-protein and RNA-ribonucleoprotein interactions by nuclease probing / Katherine A. Parker [and others]
  • Immunoprecipitation of ribonucleoproteins using autoantibodies / Joan A. Steitz, James E. Dahlberg and John N. Abelson
  • Electron microscopy of ribonucleoprotein complexes on nascent RNA using miller chromatin spreading method / Yvonne N. Osheim [and others]
  • Genetic Methods for identification and characterization of RNA-RNA and RNA-protein interactions / Roy Parker, James E. Dahlberg and John N. Abelson
  • Compilation of small nuclear RNA sequences / Ram Reddy, James E. Dahlberg and John N. Abelson
  • Sequences and classification of group I and group II introns / John M. Burke, James E. Dahlberg and John N. Abelson
  • Compilation of self-cleaving sequences from plant virus satellite RNAs and other sources / George Bruening, James E. Dahlberg and John N. Abelson.