Single molecule tools. Part B, Super-resolution, particle tracking, multiparameter and force based methods /

• Άλλοι συγγραφείς: Walter, Nils G.
• Μορφή: Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: San Diego, CA : Academic Press/Elsevier, 2010.
• Έκδοση: 1st ed.
• Σειρά: Methods in enzymology ; v. 475.
• Θέματα: Fluorescence spectroscopy.; Fluorescent probes.; Luminescence spectroscopy.; Luminescent probes.; Biosensors.; SCIENCE > Life Sciences > Molecular Biology.; Spectrometry, Fluorescence.; Fluorescence Resonance Energy Transfer.; Fluorescence.; Luminescent Measurements.; Biosensing Techniques.; Molecular Probes.; Electronic books.; Laboratory Manuals.
• Φυσική περιγραφή: 1 online resource (l, 663 pages) : illustrations (some color).
• Βιβλιογραφία: Includes bibliographical references and indexes.
• ISBN: 9780123814838; 0123814839; 9780123814821; 0123814820
• ISSN: 0076-6879 ;

Single molecule tools have begun to revolutionize the molecular sciences, from biophysics to chemistry to cell biology. They hold the promise to be able to directly observe previously unseen molecular heterogeneities, quantitatively dissect complex reaction kinetics, ultimately miniaturize enzyme assays, image components of spatially distributed samples, probe the mechanical properties of single molecules in their native environment, and "just look at the thing" as anticipated by the visionary Richard Feynman already half a century ago. This volume captures a snapshot of this vibrant, rapidly expanding field, presenting articles from pioneers in the field intended to guide both the newcomer and the expert through the intricacies of getting single molecule tools. * Includes time-tested core methods and new innovations applicable to any researcher employing single molecule tools * Methods included are useful to both established researchers and newcomers to the field * Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines.