The unfolded protein response and cellular stress. Part B /

• Άλλοι συγγραφείς: Conn, P. Michael
• Μορφή: Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: Amsterdam [Netherlands] ; Boston [Mass.] : Elsevier/Academic Press, 2011.
• Σειρά: Methods in enzymology ; v. 490.
• Θέματα: Proteins.; Protein folding.; Cellular control mechanisms.; Cell physiology.; Stress (Physiology); Cell Physiological Phenomena.; Stress, Physiological.; Electronic books.

 This volume provides descriptions of the occurrence of the UPR, methods used to assess it, pharmacological tools and other methodological approaches to analyze its impact on cellular regulation. The authors explain how these methods are able to provide important biological insights. This volume provides descriptions of the occurrence of the UPR, methods used to assess it, pharmacological tools and other methodological approaches to analyze its impact on cellular regulation. The authors explain how these methods are able to provide important biological insights. 

 Methods for investigating the UPR in filamentous fungi -- Assays for detecting the unfolded protein response -- Analysis of the role of nerve growth factor in promoting cell survival during endoplasmic reticulum stress in PC12 cells -- Measuring ER stress and the unfolded protein response using mammalian tissue culture system -- Real-time monitoring of ER stress in living cells and animals using ESTRAP assay -- HIV protease inhibitors induce endoplasmic reticulum stress and disrupt barrier integrity in intestinal epithelial cells -- Dexamethasone induction of a heat stress response -- Detecting and quantitating physiological endoplasmic reticulum stress -- PI 3-kinase regulatory subunits as regulators of the unfolded protein response -- The emerging role of histone deacetylases (HDACs) in UPR regulation -- Immunohistochemical detection of activating transcription factor 3, a hub of the cellular adaptive-response network -- Experimental approaches for elucidation of stress-sensing mechanisms of the IRE1 family proteins -- Measurement and modification of the expression level of the chaperone protein and signaling regulator GRP78/BiP in mammalian cells -- The endoplasmic reticulum-associated degradation and disulfide reductase ERdj5 -- Structural insight into the protective role of P58(IPK) during unfolded protein response -- Principles of IRE1 modulation using chemical tools -- Methods to study stromal-cell derived factor 2 in the context of ER stress and the unfolded protein response in Arabidopsis thaliana -- Nitrosative stress-induced S-glutathionylation of protein disulfide isomerase -- Methods for analyzing eIF2 kinases and translational control in the unfolded protein response.