Constitutive activity in receptors and other proteins /

• Άλλοι συγγραφείς: Conn, P. Michael
• Μορφή: Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: Amsterdam ; Boston, Mass. : Elsevier/Academic, ©2010.
• Σειρά: Methods in enzymology ; v. 484.; Methods in enzymology ; v. 485.
• Θέματα: Proteins.; Cell receptors.; SCIENCE > Life Sciences > Biochemistry.; Electronic books.

 This volume of Methods in Enzymology covers the current methodology for the detection and assessment of constitutively active proteins. The chapters written by expert authors who are leaders in the field, provide hints and tricks not available in primary research publications. It is extensively referenced, with useful figures and tables throughout the volume. A. Expert authors who are leaders in the field B. Extensively referenced and useful figures and tables C. Provides hints and tricks to facilitate reproduction of methods. 

 v. 484. Constitutive activity at the cannabinoid CB(1) receptor and behavioral responses -- Detecting constitutive activity and protean agonism at cannabinoid-2 receptor -- Modulation of the constitutive activity of the ghrelin receptor by use of pharmacological tools and mutagenesis -- Assessment of constitutive activity and internalization of GPR54 (KISS1-R) -- Assessment of constitutive activity in E-type prostanoid receptors -- [Alpha](1D-adrenergic receptors constitutive activity and reduced expression at the plasma membrane -- Constitutive activity of the histamine H(1) receptor -- Constitutive activity of somatostatin receptor subtypes -- Assessment of homologous internalization of constitutively active N111G mutant of AT(1) receptor -- Methods to detect cell surface expression and constitutive activity of GPR6 -- [beta](3)-adrenoceptor agonists and (antagonists as) inverse agonists history, perspective, constitutive activity, and stereospecific binding -- Constitutive activity of the lutropin receptor and its allosteric modulation by receptor heterodimerization -- Assessing constitutive activity of extracellular calcium-sensing receptors in vitro and in bone -- Constitutive activity of neural melanocortin receptors -- Measurement of constitutive activity of bmp type I receptors -- Probing the constitutive activity among dopamine D1 and D5 receptors and their mutants -- Identification of gain-of-function variants of the human prolactin receptor -- Investigations of activated ACVR1/ALK2, a bone morphogenetic protein type i receptor, that causes fibrodysplasia ossificans progressiva -- Identification and evaluation of constitutively active thyroid stimulating hormone receptor mutations -- Assessment of constitutive activity of a G protein-coupled receptor, CPR2, in cryptococcus neoformans by heterologous and homologous methods -- In vitro and in vivo assessment of mu opioid receptor constitutive activity -- Constitutively active [mu]-opioid receptors -- Protein kinase CK2 is a constitutively active enzyme that promotes cell survival: Strategies to identify CK2 substrates and manipulate its activity in mammalian cells -- Assessment of CK2 constitutive activity in cancer cells -- Structural basis of the constitutive activity of protein kinase CK2 -- Measuring the constitutive activation of C-jun N-terminal kinase isoforms -- Measurement of constitutive mapk and PI3K/AKT signaling activity in human cancer cell lines -- Constitutive activity of GPR40/FFA1 intrinsic or assay dependent? -- Constitutive activity of trp channels methods for measuring the activity and its outcome -- Measurement of orexin (hypocretin) and substance P effects on constitutively active inward rectifier K(+) channels in brain neurons -- Characterization of G protein-coupled receptor kinase 4 and measuring its constitutive activity in vivo -- Voltage-clamp-based methods for the detection of constitutively active acetylcholine-gated I(K, ACH) channels in the diseased heart -- Assaying WAVE and WASH complex constitutive activities toward the Arp2/3 complex -- Methods to assess the mechanism of protein function.