Animal cell culture : essential methods /

• Άλλοι συγγραφείς: Davis, John, 1952-
• Μορφή: Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: Chichester, West Sussex ; Hoboken, NJ : Wiley-Blackwell, 2011.
• Θέματα: Cell culture > Technique.; Tissue culture > Technique.; SCIENCE > Life Sciences > Cell Biology.; Electronic books.

Abstract: Animal cell culture is a widely used technology for producing recombinant proteins. The ability to make post-translational modifications and secrete the active forms of the protein into the culture medium represents major advantages over other processes. The growing market demand for pharm...

Πίνακας περιεχομένων:

• The cell culture laboratory / Sue Clarke and Janette Dillon
• Sterilization / Peter L. Roberts
• Microscopy of living cells / Colin Gray and Daniel Zicha
• Basic techniques and media, the maintenance of cell lines, and safety / John M. Davis
• Development and optimization of serum- and protein-free culture media / Stephen F. Gorfien and David W. Jayme
• Cryopreservation and banking of cell lines / Glyn N. Stacey, Ross Hawkins and Roland A. Fleck
• Primary culture of specific cell types and the establishment of cell lines / Kee Woei Ng [and others]
• Cloning / John Clarke, Alison Porter and John M. Davis
• The quality control of animal cell lines and the prevention, detection and cure of contamination / Peter Thraves and Cathy Rowe
• Systems for cell culture scale-up / Jennifer Halsall and John M. Davis
• Good laboratory practice in the cell culture laboratory / Barbara Orton.