Biothermodynamics. Part D /

• Other Authors: Johnson, Michael L., 1947-, Holt, Jo M., Ackers, Gary K.
• Format: eBook
• Language: English
• Published: Amsterdam, Netherlands ; Boston, Mass. : Elsevier/Academic Press, ©2011.
• Series: Methods in enzymology ; v. 492.
• Subjects: Thermodynamics.; Protein binding.; Protein-protein interactions.; Ligands (Biochemistry); SCIENCE > Life Sciences > Biochemistry.; Protein Binding.; Protein Array Analysis.; Ligands.; Electronic books.

The use of thermodynamics in biological research can be equated to an energy book-keeping system. While the structure and function of a molecule is important, it is equally important to know what drives the energy force. This volume presents sophisticated methods for estimating the thermodynamic par...

Table of Contents:

• A thermodynamic approach for the targeting of nucleic acid structures using their complementary single strands
• Thermodynamics of biological processes
• Protein stability in the presence of cosolutes
• Small-angle X-ray scattering studies of peptide-lipid interactions using the mouse paneth cell [alpha]-defensin cryptdin-4
• Synergy of molecular dynamics and isothermal titration calorimetry in studies of allostery
• Using tryptophan fluorescence to measure the stability of membrane proteins folded in liposomes
• Non-B conformations of CAG repeats using 2-aminopurine
• Disulfide bond-mediated passenger domain stalling as a structural probe of autotransporter outer membrane secretion in vivo
• Strategies for the thermodynamic characterization of linked binding/local folding reactions within the native state application to the lid domain of adenylate kinase from Escherichia coli
• Fluorescence-detected sedimentation in dilute and highly concentrated solutions.