Lipases. Part B, Enzyme characterization and utilization /

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More th...

Πλήρης περιγραφή

Λεπτομέρειες βιβλιογραφικής εγγραφής
Άλλοι συγγραφείς: Rubin, Byron, Dennis, Edward A.
Μορφή: Ηλ. βιβλίο
Γλώσσα:English
Έκδοση: San Diego : Academic Press, ©1997.
Σειρά:Methods in enzymology ; v. 286.
Θέματα:
Διαθέσιμο Online:Full Text via HEAL-Link
Πίνακας περιεχομένων:
  • Sect. 1. Regulation and characterization
  • Inhibition of lipid absorption as an approach to the treatment of obesity
  • Regulation of hormone-sensitive lipase activity in adipose tissue
  • Use of gene knockout mice to establish lipase function
  • Properties of pancreatic carboxyl ester lipase in mRNA-injected Xenopus oocytes and transfected mammalian hepatic and intestinal cells
  • Noncatalytic functions of lipoprotein lipase
  • Radiation inactivation studies of hepatic cholesteryl ester hydrolases
  • Immunological techniques for the characterization of digestive lipases
  • Sect. 2. Substrate and inhibitor characterization
  • Physical behavior of lipase substrates
  • Phospholipase A₂ activity and physical properties of lipid-bilayer substances
  • Covalent inactivation of lipases
  • Mechanism-based inhibitors of mammalian cholesterol esterase
  • On the inhibition of microbial lipases by tetrahydrolipstatin
  • Monolayer techniques for studying lipase kinetics
  • Recovery of monomolecular films in studies of lipolysis
  • Oil-drop tensiometer: applications for studying the kinetics of lipase action
  • A critical reevaluation of the phenomenon of interfacial activation
  • Sect. 3. Biocatalytic utility
  • Screening techniques for lipase catalyst selection
  • Kinetics, molecular modeling, and synthetic applications with microbial lipases
  • Ester synthesis via acyl transfer (transesterification)
  • Cross-linked enzyme crystals of lipases as catalysts for kinetic resolution of acids and alcohols
  • Measurement and control of hydration in non-aqueous biocatalysis
  • Solvent effect in lipase-catalyzed racemate resolution
  • Lipases in supercritical fluids
  • Stabilization of lipases against deactivation of acetaldehyde formed in acyl transfer reactions.