Protein phosphorylation. Part B, Analysis of protein phosphorylation, protein kinase inhibitors, and protein phosphatases /

• Άλλοι συγγραφείς: Hunter, Tony, 1943-, Sefton, Bartholomew M.
• Μορφή: Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: San Diego : Academic Press, ©1991.
• Σειρά: Methods in enzymology ; v. 201.
• Θέματα: Phosphoprotein phosphatases.; Protein kinases.; Protéines kinases.; Protéines.; Phosphorylation.; Phosphoprotein Phosphatases > analyse.; Protein kinases > analyse.; Protéine kinase > Inhibiteurs.; Phosphatases.; Phosphatases > Inhibiteurs.; Phosphoproteins.; Protein Kinases.; Phosphoric Monoester Hydrolases.; Electronic books.

This volume provides a compilation of recent methods for studying protein phosphorylation.

Πίνακας περιεχομένων:

• Separation of phosphotyrosine, phosphoserine, and phosphothreonine by high-performance liquid chromatography / David P. Ringer, Tony Hunter and M. Sefton Bartholomew
• Chemical properties and separation of phosphoamino acids by thin-layer chromatography and/or electrophoresis / Bertrand Duclos [and others]
• Determination of phosphoamino acid composition by acid hydrolysis of protein blotted to immobilon / Mark P. Kamps, Tony Hunter and M. Sefton Bartholomew
• Comparison of three methods for detecting tyrosine-phosphorylated photeins / Lynn M. Kozma [and others]
• Generation and use of antibodies to phosphothreonine / Daphna Heffetz [and others]
• Generation and use of anti-phosphotyrosine antibodies raised against bacterially expressed abl protein / Jean Y.J. Wang, Tony Hunter and M. Sefton Bartholomew
• Preparation and use of anti-phosphotyrosine antibodies to study structure and function of insulin receptor / Morris F. White [and others]
• Generation of monoclonal antibodies against phosphotyrosine and their use for affinity purification of phosphotyrosine-containing proteins / A. Raymond Frackelton [and others]
• Isolation of tyrosine-phosphorylated proteins and generation of monoclonal antibodies / John R. Glenney, Tony Hunter and M. Sefton Bartholomew.
• Generation and use of anti-phosphotyrosine antibodies for immunoblotting / Mark P. Kamps, Tony Hunter and M. Sefton Bartholomew
• Phosphopeptide mapping and phosphoamino acid analysis by two-dimensional separation on thin-layer cellulose plates / William J. Boyle [and others]
• Cyanogen bromide cleavage and proteolytic peptide mapping of proteins immobilized to membranes / Kunxin Luo [and others]
• Analysis of the in vivo phosphorylation states of proteins by fast atom bombardment mass spectrometry and other techniques / Philip Cohen [and others]
• Determination and location of phosphoserine in proteins and peptides by conversion to S-ethylcysteine / Helmut E. Meyer [and others]
• Solid-phase sequencing of 32P-labeled phosphopeptides at picomole and subpicomole levels / Richard E.H. Wettenhall [and others]
• Identification of phosphorylation sites in peptides using a microsequencer / Peter T. Roach [and others]
• Sequence analysis of phosphotyrosine-containing peptides / Helmut E. Meyer [and others]
• Synthesis of O-phosphoserine- and O-phosphothreonine-containing peptides / John W. Perich, Tony Hunter and M. Sefton Bartholomew
• Synthesis of O-phosphotyrosine-containing peptides / John W. Perich, Hunter Tony and M. Sefton Bartholomew
• Measurement of stoichiometry of protein phosphorylation by biosynthetic labeling / Bartholomew M. Sefton, Tony Hunter and M. Sefton Bartholomew
• Estimation of phosphorylation stoichiometry by separation of phosphorylated isoforms / Jonathan Acooper, Tony Hunter and M. Sefton Bartholomew
• Microchemical determination of phosphate in proteins isolated from polyacrylamide gels / Victor Dvacquier [and others]
• Production of phosphorylation state-specific antibodies / Andrew Jczernik [and others].
• Pseudosubstrate-based peptide inhibitors / Bruce E. Kemp [and others]
• Utilization of the inhibitor protein of adenosine cyclic monophosphate-dependent protein kinase, and peptides derived from it, as tools to study adenosine cyclic monophosphate-mediated cellular processes / Donal Awalsh [and others]
• Use of sphingosine as inhibitor of protein kinase C / Yusuf A. Hannun [and others]
• Properties and use of H-series compounds as protein kinase inhibitors / Hiroyoshi Hidaka [and others]
• Use and specificity of staurosporine, UCN-O1, and calphostin C as protein kinase inhibitors / Tatsuya Tamaoki, Tony Hunter and M. Sefton Bartholomew
• Inhibition of protein-tyrosine kinases by tyrphostins / Alexander Levitzki [and others]
• Use and specificity of genistein as inhibitor of protein-tyrosine kinases / Tetsu Akiyama [and others]
• Use and selectivity of herbimycin A as inhibitor of protein-tyrosine kinases / Yoshimasa Uehara [and others]
• Use of erbstatin as protein-tyrosine kinase inhibitor / Kazuo Umezawa [and others]
• Classification of protein- serine/threonine phosphatases: identification and quantitation in cell extracts / Philip Cohen, Tony Hunter and M. Sefton Bartholomew
• Cloning of protein- serine/threonine phosphatases / Patricia T.W. Cohen, Hunter Tony and M. Sefton Bartholomew
• Reactivation of protein phosphatase 1 expressed at high levels from recombinant baculovirus / Patricia T.W. Cohen [and others]
• Targeting subunits for protein phosphatases / Michael J. Hubbard [and others]
• Purification of protein-tyrosine phosphatases from human placenta / Nicholas K. Tonks [and others]
• Purification and assay of CD45: An integral membrane protein-tyrosine phosphatase / Nicholas K. Tonks [and others]
• Resolution and characterization of multiple protein-tyrosine phosphatase activities / Thomas S. Ingebritsen, Tony Hunter and M. Sefton Bartholomew
• Use of okadaic acid to inhibit protein phosphatases in intact cells / D. Grahame Hardie [and others]
• Use of vanadate as protein-phosphotyrosine phosphatase inhibitor / Julius A. Gordon, Tony Hunter and M. Sefton Bartholomew.