Guide to molecular cloning techniques /

• Other Authors: Berger, Shelby L., Kimmel, Alan R.
• Format: eBook
• Language: English
• Published: Orlando : Academic Press, 1987.
• Series: Methods in enzymology ; v. 152.
• Subjects: Molecular cloning.; Clonage moléculaire > Technique.; Clonage > Technique.; ADN.; Clonage.; ADN = Acide désoxyribonucléique.; Génétique.; Manuels.; Kloneren.; Genklonierung; Aufsatzsammlung; Klonierung; Nucleinsäuren; Methode; Cloning, Molecular > methods.; DNA, Recombinant.; Biologia molecular; Laboratorio; Acido nucleico; DNA recombinante; Bacteria; Clonagem; RNA; Molecular biology; Laboratory; Nucleic acids; Recombinant DNA; Cloning; Clones; Electronic books.
• Physical Description: 1 online resource (xl, 812 pages) : illustrations.
• Format: Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.
• Bibliography: Includes bibliographical references and index.
• ISBN: 9780121820534; 012182053X; 012181775X; 9780121817756

Requirements for a molecular biology laboratory. General methods for isolating and characterizing nucleic acids. Enzymatic techniques and recombinant DNA technology. Restriction enzymes. Growth and maintenance of bacteria. Genomic cloning. Preparation and characterization of RNA. Preparation of cDNA and the generation of cDNA libraries. Selection of clones from libraries. Identification and characterization of specific clones.