Nucleosomes, histones & chromatin. Part A /

• Άλλοι συγγραφείς: Wu, Carl, Allis, C. David
• Μορφή: Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: Amsterdam, Netherland ; Boston, Mass. : Elsevier/Academic Press, ©2012.
• Σειρά: Methods in enzymology ; v. 512.
• Θέματα: Nucleoproteins.; Histones.; Chromatin.; SCIENCE > Life Sciences > Molecular Biology.; Nucleosomes.; Electronic books.

 This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. The first of 2 volumes covering Nucleosomes, Histones & Chromatin and has chapters on Methods applied to the study of Protein Arginine Methylation, High resolution identification of intra- and interchromosomal DNA interactions by 4C technology, and Peptide arrays to interrogate the binding specificity of chromatin-binding proteins. Continues the legacy of this premier serial by containing quality chapters authored by leaders in the field The first of 2 volumes covering nucleosomes, histones and chromatin Chapters on methods applied to the study of protein arginine methylation, high-resolution identification of intra- and interchromosomal DNA interactions by 4C technology, and peptide arrays to interrogate the binding specificity of chromatin-binding proteins. 

 Examining histone posttranslational modification patterns by high-resolution mass spectrometry -- Investigating the cell cycle-associated dynamics of histone modifications using quantitative mass spectrometry -- A method to site-specifically incorporate methyl-lysine analogues into recombinant proteins -- Methods applied to the study of protein arginine methylation -- Enzymatic analysis of Tet proteins: key enzymes in the metabolism of DNA methylation -- Peptide mciroarrays to interrogate the "histone code" -- Identifying chromatin readers using a SILAC-based histone peptide pull-down approach -- High-throughput strategy to identify inhibitors of histone-binding domains -- Validation of histone-binding partners by peptide pull-downs and isothermal titration calorimetry -- Analysis of histone chaperone antisilencing function 1 interactions -- Quantifying chromatin-associated interactions: the HI-FI system -- Biochemical assay for histone H2A.Z replacement by the yeast SWR1 chromatin remodeling complex -- Experimental analysis of the mechanism of chromatin remodeling by RNA polymerase II.