Nucleosomes, histones & chromatin. Part A /

• Other Authors: Wu, Carl, Allis, C. David
• Format: eBook
• Language: English
• Published: Amsterdam, Netherland ; Boston, Mass. : Elsevier/Academic Press, ©2012.
• Series: Methods in enzymology ; v. 512.
• Subjects: Nucleoproteins.; Histones.; Chromatin.; SCIENCE > Life Sciences > Molecular Biology.; Nucleosomes.; Electronic books.

This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. The first of 2 volumes covering Nucleosomes, Histones & Chromatin and has chapters on Methods applied to the study of Protein Arginine Methylation...

Table of Contents:

• Examining histone posttranslational modification patterns by high-resolution mass spectrometry
• Investigating the cell cycle-associated dynamics of histone modifications using quantitative mass spectrometry
• A method to site-specifically incorporate methyl-lysine analogues into recombinant proteins
• Methods applied to the study of protein arginine methylation
• Enzymatic analysis of Tet proteins: key enzymes in the metabolism of DNA methylation
• Peptide mciroarrays to interrogate the "histone code"
• Identifying chromatin readers using a SILAC-based histone peptide pull-down approach
• High-throughput strategy to identify inhibitors of histone-binding domains
• Validation of histone-binding partners by peptide pull-downs and isothermal titration calorimetry
• Analysis of histone chaperone antisilencing function 1 interactions
• Quantifying chromatin-associated interactions: the HI-FI system
• Biochemical assay for histone H2A.Z replacement by the yeast SWR1 chromatin remodeling complex
• Experimental analysis of the mechanism of chromatin remodeling by RNA polymerase II.