Lipidomics technologies and applications /

• Άλλοι συγγραφείς: Ekroos, Kim
• Μορφή: Ηλεκτρονική πηγή Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: Weinheim : Wiley-VCH, [2012]
• Θέματα: Biochemical markers > Diagnostic use.; Lipids > Analysis.; Lipids > analysis.; Biomarkers > analysis.; Lipid Metabolism.; SCIENCE / Life Sciences / Biochemistry; Lipidomik; Electronic books.

 Machine generated contents note: 1.Lipidomics Perspective: From Molecular Lipidomics to Validated Clinical Diagnostics / Kim Ekroos -- 1.1.Introduction -- 1.2.Hierarchical Categorization of the Analytical Lipid Outputs -- 1.2.1.Lipid Class -- 1.2.2.Sum Compositions -- 1.2.3.Molecular Lipids -- 1.2.4.Structurally Defined Molecular Lipids -- 1.3.The Type of Lipid Information Delivers Different Biological Knowledge -- 1.4.Untying New Biological Evidences through Molecular Lipidomic Applications -- 1.5.Molecular Lipidomics Approaches Clinical Diagnostics -- 1.6.Current Roadblocks in Lipidomics -- 1.7.Conclusions -- References -- 2.Lipids in Cells / Michal Surma -- 2.1.Introduction -- 2.2.Basis of Cellular Lipid Distribution -- 2.3.Lipid Distribution by Nonvesicular Routes -- 2.4.Lipids in Different Cell Types -- 2.5.Functional Implications of Membrane Lipid Composition -- 2.6.Outlook: Collectives and Phase Separation -- References -- 3.High-Throughput Molecular Lipidomics / Kim Ekroos -- 3.1.Introduction -- 3.2.Lipid Diversity -- 3.3.Function of Molecular Lipids -- 3.4.Automated Sample Preparation -- 3.5.Different Approaches to Molecular Lipidomics -- 3.5.1.Untargeted versus Targeted Approaches -- 3.5.2.Shotgun Lipidomics -- 3.5.3.Analytical Validation of the Shotgun Approach -- 3.5.4.Targeted LC-MS Lipidomics -- 3.6.Data Processing and Evaluation -- 3.7.Lipidomic Workflows -- 3.8.Conclusions and Future Perspectives -- References -- 4.Multidimensional Mass Spectrometry-Based Shotgun Lipidomics / Xianlin Han -- 4.1.Introduction -- 4.2.Multidimensional Mass Spectrometry-Based Shotgun Lipidomics -- 4.2.1.Intrasource Separation -- 4.2.2.The Principle of Multidimensional Mass Spectrometry -- 4.2.3.Variables in Multidimensional Mass Spectrometry -- 4.2.3.1.Variables in Fragment Monitoring by Tandem MS Scans -- 4.2.3.2.Variables Related to the Infusion Conditions -- 4.2.3.3.Variables under Ionization Conditions -- 4.2.3.4.Variables under Collision Conditions -- 4.2.3.5.Variables Related to the Sample Preparations -- 4.3.Application of Multidimensional Mass Spectrometry-Based Shotgun Lipidomics for Lipidomic Analysis -- 4.3.1.Identification of Lipid Molecular Species by 2D Mass Spectrometry -- 4.3.1.1.Identification of Anionic Lipids -- 4.3.1.2.Identification of Weakly Anionic Lipids -- 4.3.1.3.Identification of Charge Neutral but Polar Lipids -- 4.3.1.4.Identification of Sphingolipids -- 4.3.1.5.The Concerns of the MDMS-Based Shotgun Lipidomics for Identification of Lipid Species -- 4.3.2.Quantification of Lipid Molecular Species by MDMS-Based Shotgun Lipidomics -- 4.3.2.1.The Principle of Quantification of Individual Lipid Species by MS -- 4.3.2.2.Quantification by Using a Two-Step Procedure in MDMS-Based Shotgun Lipidomics -- 4.3.2.3.Quantitative Analysis of PEX7 Mouse Brain Lipidome by MDMS-Based Shotgun Lipidomics -- 4.4.Conclusions -- References -- 5.Targeted Lipidomics: Sphingolipidomics / M. Cameron Sullards -- 5.1.Introduction -- 5.2.Sphingolipids Description and Nomenclature -- 5.3.Sphingolipids Analysis via Targeted LC-MS/MS -- 5.3.1.Sphingolipid Internal Standards -- 5.3.2.Biological Sample Preparation and Storage -- 5.3.3.Sphingolipid Extraction Protocol -- 5.3.4.Liquid Chromatography -- 5.3.4.1.LCBs and Cer1P -- 5.3.4.2.Cer, HexCer, LacCer, SM, ST, and Cer1P -- 5.3.4.3.Separation of GlcCer and GalCer -- 5.3.5.Mass Spectrometry -- 5.3.5.1.Electrospray Ionization -- 5.3.5.2.Tandem Mass Spectrometry -- 5.3.5.3.Multiple Reaction Monitoring -- 5.3.6.Generation of Standard Curves -- 5.3.7.Data Analysis -- 5.3.8.Quality Control -- 5.4.Applications of Sphingolipidomics in Biology and Disease -- 5.4.1.LC-MS/MS -- 5.4.2.Transcriptomic Guided Tissue Imaging Mass Spectrometry -- 5.5.Conclusions -- References -- 6.Structural Lipidomics / Stephen J. Blanksby -- 6.1.Introduction -- 6.2.Lipid Structure -- 6.3.Structural Analysis of Lipids by Mass Spectrometry -- 6.4.sn Position -- 6.5.Double Bond Position -- 6.5.1.Untargeted Fragmentation -- 6.5.2.Targeted Fragmentation -- 6.6.Double Bond Stereochemistry -- 6.7.Conclusions -- References -- 7.Imaging Lipids in Tissues by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry / Robert C. Murphy -- 7.1.Introduction -- 7.2.Sample Preparation -- 7.3.Matrix -- 7.3.1.Techniques for Matrix Application -- 7.3.2.Matrix Compounds -- 7.4.Instrumentation -- 7.4.1.Lasers and Rastering -- 7.4.2.Ion Formation -- 7.4.3.Mass Analyzers and Ion Detection -- 7.5.Data Processing -- 7.6.Conclusions -- References -- 8.Lipid Informatics: From a Mass Spectrum to Interactomics / Kirill Tarasov -- 8.1.Introduction -- 8.2.Lipid Nomenclature -- 8.3.Basic Properties of Lipid Mass spectrometric Data -- 8.3.1.Mass Spectrum -- 8.3.2.Mass Accuracy and Reproducibility -- 8.3.3.Isotopes, Deisotoping, and Isotope Correction -- 8.4.Data Processing -- 8.4.1.De Novo Lipid Identification -- 8.4.2.Targeted Export of Lipidomic Data -- 8.4.3.Normalization of lipidomic Data -- 8.5.Lipidomic Data Mining and Visualization -- 8.5.1.Comparative Lipidomics -- 8.5.2.Multivariate Data Analysis -- 8.5.3.Lipidomics in Biomarker Research -- 8.6.Lipidomic Data Integration -- 8.7.Conclusions and Future Perspectives -- References -- 9.Lipids in Human Diseases / Scott A.