G protein coupled receptors. Structure /

• Άλλοι συγγραφείς: Conn, P. Michael
• Μορφή: Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: San Diego, Calif. : Academic Press/Elsevier, 2013.
• Σειρά: Methods in enzymology ; v. 520.
• Θέματα: G proteins > Receptors.; SCIENCE > Life Sciences > Biochemistry.; Receptors, G-Protein-Coupled.; Electronic books.

This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. This volume covers G protein coupled receptors and includes chapters on such topics as post-translation modification of GPCR in relationship to biased...

Πίνακας περιεχομένων:

• Expression of GPCRs in Pichia pastoris for structural studies
• Conformational ensemble view of G protein-couples receptors and the effect of mutations and ligand binding
• Structural evolution of G-protein-coupled receptors: a sequence space approach
• Directed evolution of G-protein-coupled receptors for high functional expression and detergent stability
• The role of hydrophobic amino acids in the structure and function of the Rhodopsin family of G protein-coupled receptors
• Structure of [beta]-adrenergic receptors
• Advances in methods to characterize ligand-induced ionic lock and Rotamer toggle molecular switch in G protein-coupled receptors
• Crystallogenesis of adenosine A2A receptor-T4 lysozyme fusion protein: a practical route for the structure
• Probing GPCR structure: adenosine and P2Y nucleotide receptors
• Strategies for studying the ligand binding site of GPCRs: photoaffinity labeling of the VPAC1 receptor, a prototype of class B GPCRs
• Expression of mammalian G protein-coupled receptors in Caenorhabditis elegans
• Expression, purification, and structural analysis of intracellular C-termini from metabotropic glutamate receptors
• Unnatural amino acid mutagenesis of GPCRs using amber codon suppression and bioorthogonal labeling
• Mapping a ligand binding site using genetically encoded photoactivatable crosslinkers
• Alternative mRNA splicing of G protein-coupled receptors
• Functional residues essential for the activation of the C1 Cannabinoid receptor.