Methods in protein design. Volume 523 /

• Άλλοι συγγραφείς: Keating, Amy E.
• Μορφή: Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: San Diego, Calif. : Academic, 2013.
• Σειρά: Methods in enzymology ; volume 523.
• Θέματα: Protein engineering.; SCIENCE > Biotechnology.; Electronic books.

 Computational design of novel protein binders and experimental affinity maturation -- Mining tertiary structural motifs for assessment of designability -- Computational methods for controlling binding specificity -- Flexible backbone sampling methods to model and design protein alternative conformations -- Osprey: protein design with ensembles, flexibility, and provable algorithms -- Scientific benchmarks for guiding macromolecular energy function improvement -- Molecular dynamics simulations for the ranking, evaluation, and refinement of computationally designed proteins -- Multistate protein design using CLEVER and CLASSY -- Using analyses of amino acid coevolution to understand protein structure and function -- Evolution-based design of proteins -- Protein engineering and stabilization from sequence statistics: variation and covariation analysis -- Enzyme engineering by targeted libraries -- Generation of high-performance binding proteins for pepetide motifs by affinity clamping -- Engineering fibronectin-based binding proteins by yeast surface display -- Engineering and analysis of peptide-recognition domain specificities by phage display and deep sequencing -- Efficient sampling of SCHEMA chimera families to identify useful sequence elements -- Protein switch engineering by domain insertion -- Design of chimeric proteins by combination of subdomain-sized fragments -- [alpha]-helix mimicry with [alpha/beta]-peptides.