Methods in protein design. Volume 523 /

• Άλλοι συγγραφείς: Keating, Amy E.
• Μορφή: Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: San Diego, Calif. : Academic, 2013.
• Σειρά: Methods in enzymology ; volume 523.
• Θέματα: Protein engineering.; SCIENCE > Biotechnology.; Electronic books.

Πίνακας περιεχομένων:

• Computational design of novel protein binders and experimental affinity maturation
• Mining tertiary structural motifs for assessment of designability
• Computational methods for controlling binding specificity
• Flexible backbone sampling methods to model and design protein alternative conformations
• Osprey: protein design with ensembles, flexibility, and provable algorithms
• Scientific benchmarks for guiding macromolecular energy function improvement
• Molecular dynamics simulations for the ranking, evaluation, and refinement of computationally designed proteins
• Multistate protein design using CLEVER and CLASSY
• Using analyses of amino acid coevolution to understand protein structure and function
• Evolution-based design of proteins
• Protein engineering and stabilization from sequence statistics: variation and covariation analysis
• Enzyme engineering by targeted libraries
• Generation of high-performance binding proteins for pepetide motifs by affinity clamping
• Engineering fibronectin-based binding proteins by yeast surface display
• Engineering and analysis of peptide-recognition domain specificities by phage display and deep sequencing
• Efficient sampling of SCHEMA chimera families to identify useful sequence elements
• Protein switch engineering by domain insertion
• Design of chimeric proteins by combination of subdomain-sized fragments
• [alpha]-helix mimicry with [alpha/beta]-peptides.