Laboratory methods in enzymology. DNA / Volume 529 :
Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols. In this volume, we have brought together a number of core protocols concentrating on DNA, complementi...
Άλλοι συγγραφείς: | |
---|---|
Μορφή: | Ηλ. βιβλίο |
Γλώσσα: | English |
Έκδοση: |
Amsterdam ; Boston :
Elsevier/Academic Press,
[2013]
|
Έκδοση: | First edition. |
Σειρά: | Methods in enzymology ;
v. 529. |
Θέματα: | |
Διαθέσιμο Online: | Full Text via HEAL-Link |
Πίνακας περιεχομένων:
- Explanatory chapter: PCR primer design / Rubén Álvarez-Fernández
- Explanatory chapter: How plasmid preparation kits work / Laura Koontz
- Explanatory chapter: Introducing exogenous DNA into cells / Laura Koontz
- Agarose gel electrophoresis / Laura Koontz
- Analysis of DNA by southern blotting / Gary Glenn and Lefkothea-Vasiliki Andreou
- Purification of DNA oligos by denaturing polyacrylamide gel electrophoresis (PAGE) / Sara Lopez-Gomollon and Francisco Esteban Nicolas
- Molecular cloning / Juliane C. Lessard
- Rapid creation of stable mammalian cell lines for regulated expression of proteins using the Gateway® recombination cloning technology and Flp-In T-REx® lines / Jessica Spitzer, Markus Landthaler, and Thomas Tuschl
- Restrictionless cloning / Mikkel A. Algire
- Isolation of plasmid DNA from bacteria / Lefkothea-Vasiliki Andreou
- Preparation of genomic DNA from bacteria / Lefkothea-Vasiliki Andreou
- Preparation of genomic DNA from Saccharomyces cerevisiae / Jessica S. Dymond
- Isolation of genomic DNA from mammalian cells / Cheryl M. Koh
- Sanger dideoxy sequencing of DNA / Sarah E. Walker and Jon Lorsch
- Preparation of fragment libraries for next-generation sequencing on the applied biosystems SOLiD platform / Srinivasan Yegnasubramanian
- Explanatory chapter: Next generation sequencing / Srinivasan Yegnasubramanian
- Generating mammalian stable cell lines by electroporation / Patti A. Longo, Jennifer M. Kavran, Min-Sung Kim, and Daniel J. Leahy
- Transient mammalian cell transfection with polyethylenimine (PEI) / Patti A. Longo, Jennifer M. Kavran, Min-Sung Kim, and Daniel J. Leahy
- Site-directed mutagenesis / Julia Bachman
- PCR-based random mutagenesis / Jessica S. Dymond
- Megaprimer method for mutagenesis of DNA / Craig W. Vander Kooi
- Explanatory chapter: Troubleshooting PCR / Kirstie Canene-Adams
- Explanatory chapter: Quantitative PCR / Jessica S. Dymond
- General PCR / Kirstie Canene-Adams
- Colony PCR / Megan Bergkessel and Christine Guthrie
- Chemical transformation of yeast / Megan Bergkessel and Christine Guthrie
- Transformation of E. coli via electroporation / Juliane C. Lessard
- Transformation of chemically competent E. coli / Rachel Green and Elizabeth J. Rogers.