Laboratory methods in enzymology. Volume 530, RNA /

• Άλλοι συγγραφείς: Lorsch, Jon (Επιμελητής έκδοσης)
• Μορφή: Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: Amsterdam ; Boston : Elsevier/Academic Press, 2013.
• Έκδοση: First edition.
• Σειρά: Methods in enzymology ; v. 530.
• Θέματα: Enzymes.; Biochemistry > Technique.; Biotechnology > Methodology.; Molecular biology > Methodology.; SCIENCE > Life Sciences > Biochemistry.; Sequence Analysis, RNA > methods.; Biotechnology > methods.; Biotechnology > istrumentation.; Molecular Biology > methods.; Molecular Biology > istrumentation.; Electronic books.

Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols. In this volume, we have brought together a number of core protocols concentrating on RNA, complementi...

Πίνακας περιεχομένων:

• Computational prediction of RNA secondary structure / Walter N. Moss
• Reverse-transcription PCR (RT-PCR) / Julia Bachman
• Northern blotting / Shan L. He and Rachel Green
• Explanatory chapter: nuclease protection assays / Elizabeth Eyler
• In vitro transcription from plasmid or PCR-amplified DNA / Julie L. Brunelle and Rachel Green
• Measurement of in vivo RNA synthesis rates / David S. McPheeters and Jo Ann Wise
• Method for measuring mRNA decay rate in Saccharomyces cerevisiae / Wenqian Hu and Jeff Coller
• Analysis of polysomes from bacteria / Daoming Qin and Kurt Fredrick
• Polysome profile analysis : yeast / Martin Pospísek and Leos Valásek
• Polysome analysis of mammalian cells / Shan L. He and Rachel Green
• Polysome analysis for determining mRNA and ribosome association in Saccharomyces cerevisiae / Wenqian Hu and Jeff Coller
• Oligo(dT)-primed RT-PCR isolation of polyadenylated RNA degradation intermediates / Shimyn Slomovic and Gadi Schuster
• Circularized RT-PCR (cRT-PCR) : analysis of the 5' ends, 3' ends, and poly(A) tails of RNA / Shimyn Slomovic and Gadi Schuster
• RNA radiolabeling / Rishi Porecha and Daniel Herschlag
• Fluorescently labeling synthetic RNAs / Max Greenfield and Daniel Herschlag
• Analysis of RNA by analytical polyacrylamide gel electrophoresis / Alexey Petrov, Albet Tsa, and Joseph D. Puglisi
• RNA purification by preparative polyacrylamide gel electrophoresis / Alexey Petrov, Tinghe Wu, Elisabetta Viani Puglisi, and Joseph D. Puglisi
• Explanatory chapter : nucleic acid concentration determination / Sarah Kolitz and Jon Lorsch
• RNA purification : precipitation methods / Sarah E. Walker and Jon Lorsch
• Reverse transcriptase dideoxy sequencing of RNA / Sarah E. Walker and Jon Lorsch
• RNA structure experimental analysis : chemical modification / Zhili Xu and Gloria Culver
• Structural analysis of RNA backbone using in-line probing / Ali Nahvi and Rachel Green.