Laboratory methods in enzymology. Volume 539, Protein, Part B /

• Άλλοι συγγραφείς: Lorsch, Jon (Επιμελητής έκδοσης)
• Μορφή: Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: San Diego, California : Academic Press, 2014.
• Σειρά: Methods in enzymology ; volume 539, pt. B.
• Θέματα: Biotechnology > Methodology.; Biotechnology.; Proteins > Analysis.; Enzymes > Analysis.; SCIENCE > Essays.; SCIENCE > Reference.; Proteins > analysis.; Electronic books.

Laboratory Methods in Enzymology: Protein Part B brings together a number of core protocols concentrating on protein, carefully written and edited by experts. Indispensable tool for the researcher Carefully written and edited by experts to contain step-by-step protocolsIn this volume we have brought...

Πίνακας περιεχομένων:

• In vitro synthesis of proteins in bacterial extracts
• Preparation of a Saccharomyces cerevisiae cell-free extract for in vitro translation
• Yeast two-hybrid screen
• UV cross-linking of interacting RNA and protein in cultured cells
• Analysis of RNA-protein interactions by cell mixing
• General protein-protein cross-linking
• Chromatin immunoprecipitation and multiplex sequencing (ChIP-Seq) to identify global transcription factor binding sites in the nematode Caenorhabditis elegans
• PAR-CLIP (phoactivatable ribonucleoside-enhanced crosslinking and immunoprecipitation): a step-by-step protocol to the transcriptome-wide identification of binding sites of RNA-binding proteins
• Determining the RNA specificity and targets of RNA-binding proteins using a three-hybrid system
• Dissecting a known RNA-protein interaction using a yeast three-hybrid system
• Identifying proteins that bind a known RNA sequence using the yeast three-hybrid system.