Reconstituting the cytoskeleton /

• Άλλοι συγγραφείς: Vale, Ronald (Επιμελητής έκδοσης), Akhmanova, Anna (Συντελεστής)
• Μορφή: Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: San Diego, California : Academic Press, 2014.
• Έκδοση: First edition.
• Σειρά: Methods in enzymology ; v. 540.
• Θέματα: Cytoskeleton.; Microtubules.; Muscle proteins.; Clinical enzymology.; HEALTH & FITNESS > Diseases > General.; MEDICAL > Clinical Medicine.; MEDICAL > Diseases.; MEDICAL > Evidence-Based Medicine.; MEDICAL > Internal Medicine.; Muscle Proteins.; Electronic books.; Ebook.

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers cytoskeletal structure, including such topics as rotational movement of formins studied by fluorescence polarization microscopy, in vitro as...

Πίνακας περιεχομένων:

• Actin filament dynamics using microfluidics
• Bacterial actin-like proteins: purification and characterization of self-assembly properties
• Quantitative analysis of microtubule self-assembly kinetics and tip structure
• Biochemical reconstitution of the WAVE regulatory complex
• Rotational movement of formins evaluated by using single-molecule fluorescence polarization
• Single-molecule studies of actin assembly and disassembly factors
• Assaying microtubule nucleation of the [gamma]-Tubulin ring complex
• Reconstituting dynamic microtubule polymerization regulation by TOG domain proteins
• Generation of differentially modified microtubules using In Vitro enzymatic approaches
• Engineering defined motor ensembles with DNA origami
• Construction and analyses of elastically coupled multiple-motor systems
• Reconstitution of cortical dynein function
• Reconstitution of microtubule-dependent organnelle transport
• Reconstituting the motility of isolated intracellular cargoes
• Reconstitution of contractile actomyosin arrays
• Directed actin assembly and motility
• In Vitro reconstitution of dynamic microtubules interacting with actin filament networks
• Measuring kinetochore-microtubule interaction In Vitro
• Micropattern-guided assembly of overlapping pairs of dynamic microtubules
• WAVE regulatory complex activation
• Dissecting principles governing actin assembly using yeast extracts
• Xenopus egg ctyoplasm with intact actin
• Glycogen-supplemented mitotic cytosol for analyzing xenopus egg microtubule organization
• Spindle assembly on immmobilized chromatin micropatterns.