Laboratory methods in enzymology.

Laboratory methods in enzymology. Volume 541, Protein, Part C /

In this volume we have brought together a number of core protocols concentrating on Protein, carefully written and edited by experts.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Άλλοι συγγραφείς: Lorsch, Jon (Επιμελητής έκδοσης)
Μορφή: Ηλ. βιβλίο
Γλώσσα:English
Έκδοση: Amsterdam : Elsevier/Academic Press, 2014.
Σειρά:Methods in enzymology ; volume 541.
Θέματα:
Proteins > Analysis.
Proteins > Purification.
SCIENCE > Life Sciences > Biochemistry.
Electronic books.
Διαθέσιμο Online:Full Text via HEAL-Link
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245 0 0 |a Laboratory methods in enzymology.  |n Volume 541,  |p Protein,  |n Part C /  |c edited by Jon Lorsch. 
246 3 |a Protein 
264 1 |a Amsterdam :  |b Elsevier/Academic Press,  |c 2014. 
300 |a 1 online resource (xvii, 272 pages) :  |b illustrations (black and white). 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
338 |a online resource  |b cr  |2 rdacarrier 
490 1 |a Methods in enzymology,  |x 0076-6879 ;  |v volume 541 
504 |a Includes bibliographical references and index. 
520 |a In this volume we have brought together a number of core protocols concentrating on Protein, carefully written and edited by experts. 
505 0 |a Front Cover; Laboratory Methods in Enzymology: Protein Part C; Copyright; Contents; Contributors; Miscellaneous; Preface; Section I: Protein Protocols/Protein Precipitation; Chapter One: TCA Precipitation; 1. Theory; 2. Equipment; 3. Materials; 3.1. Solutions and buffers; 4. Protocol; 4.1. Preparation; 4.2. Duration; 4.3. Caution; 5. Step 1A Trichloroacetic Acid Precipitation; 5.1. Overview; 5.2. Duration; 5.3. Tip; 5.4. Tip; 5.5. Tip; 6. Step 1B Deoxycholate-Trichloroacetic Acid Precipitation; 6.1. Overview; 6.2. Duration; 6.3. Tip; 6.4. Tip; 6.5. Tip; References. 
505 8 |a Referenced Protocols in Methods NavigatorSection II: Protein Protocols/Protein Pull-Down Methods; Chapter Two: Coimmunoprecipitation of Proteins from Yeast; 1. Theory; 2. Equipment; 3. Materials; 3.1. Solutions and buffers; 4. Protocol; 4.1. Duration; 4.2. Preparation; 5. Step 1 Preparation of Whole Cell Lysates; 5.1. Overview; 5.2. Duration; 6. Step 2 Normalization of Cell Lysates; 6.1. Overview; 6.2. Duration; 6.3. Tip; 7. Step 3 Coimmunoprecipitation; 7.1. Overview; 7.2. Duration; 7.3. Tip; 7.4. Tip; 7.5. Tip; 7.6. Tip; 7.7. Tip; 7.8. Tip; 7.9. Note. 
505 8 |a 8. Step 4 Wash and Elute the Immunoprecipitates8.1. Overview; 8.2. Duration; 8.3. Tip; 8.4. Tip; 9. Step 5 Analysis of Immunoprecipitations; 9.1. Overview; 9.2. Duration; References; Referenced Literature; Related Literature; Referenced Protocols in Methods Navigator; Chapter Three: Coupling Antibody to Cyanogen Bromide-Activated Sepharose; 1. Theory; 2. Equipment; 3. Materials; 3.1. Solutions and buffers; 4. Protocol; 4.1. Duration; 4.2. Preparation; 5. Step 1 Preparation of Antibody and Resin; 5.1. Overview; 5.2. Duration; 5.3. Tip; 5.4. Tip; 5.5. Tip; 5.6. Tip. 
505 8 |a 6. Step 2 Coupling the Antibody to the Resin6.1. Overview; 6.2. Duration; 6.3. Tip; 7. Step 3 Quench the Reaction; 7.1. Overview; 7.2. Duration; 7.3. Tip; 8. Step 4 Wash the Resin; 8.1. Overview; 8.2. Duration; References; Referenced Protocols in Methods Navigator; Chapter Four: Analysis of Protein-Protein Interactions by Coimmunoprecipitation; 1. Theory; 2. Equipment; 3. Materials; 3.1. Solutions and buffers; 4. Protocol; 4.1. Preparation; 4.2. Duration; 5. Step 1 Isolation of the Protein of Interest by Immunoprecipitation; 5.1. Overview; 5.2. Duration; 5.3. Tip; 5.4. Tip; 5.5. Tip. 
505 8 |a 6. Step 2 Detection of the Binding Partner by Immunoblotting6.1. Overview; 6.2. Duration; 6.3. Tip; 6.4. Tip; References; Referenced Literature; Referenced Protocols in Methods Navigator; Section III: Protein Protocols/Protein Purification; Chapter Five: Use and Application of Hydrophobic Interaction Chromatography for Protein Purification; 1. Theory; 1.1. Latest technology in HIC adsorbents; 1.2. Advantages and disadvantages of using HIC; 2. Equipment; 3. Materials; 3.1. Solutions and buffers; 3.2. Preparation; 4. Protocol; 4.1. Preparation; 4.2. Duration; 5. Step 1 Column Equilibration. 
650 0 |a Proteins  |x Analysis. 
650 0 |a Proteins  |x Purification. 
650 7 |a SCIENCE  |x Life Sciences  |x Biochemistry.  |2 bisacsh 
650 7 |a Proteins  |x Analysis.  |2 fast  |0 (OCoLC)fst01079714 
650 7 |a Proteins  |x Purification.  |2 fast  |0 (OCoLC)fst01079750 
655 4 |a Electronic books. 
700 1 |a Lorsch, Jon,  |e editor. 
776 0 8 |i Print version:  |a Lorsch, Jon.  |t Laboratory Methods in Enzymology.  |d Academic Press 2014  |z 1306540887 
830 0 |a Methods in enzymology ;  |v volume 541.  |x 1557-7988 
856 4 0 |u https://www.sciencedirect.com/science/bookseries/00766879/541  |z Full Text via HEAL-Link 

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