Cell-wide metabolic alterations associated with malignancy /

• Άλλοι συγγραφείς: Galluzzi, Lorenzo, Kroemer, Guido
• Μορφή: Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: Amsterdam ; Boston : Academic Press, 2014.
• Έκδοση: First edition.
• Σειρά: Methods in enzymology ; v. 543.
• Θέματα: Biochemistry.; Cancer.; Enzymes.; SCIENCE > Life Sciences > Biology.; Neoplasms.; Metabolism.; Electronic books.

 This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods providing a theoretical overview on metabolic alterations of cancer cells and a series of protocols that can be employed to study oncometabolism, in vitro, ex vivo and in vivo. Malignant cells exhibit metabolic changes when compared to their normal counterparts, owing to both genetic and epigenetic alterations. Although such a metabolic rewiring has recently been indicated as ""yet another"" general hallmark of canc. 

 Methods to measure cytoplasmic and mitochondrial Ca2+ concentration using Ca2+ -sensitive dyes -- Methods to measure intracellular Ca2+ fluxes with organelle-targeted aequorin-bsaed probes -- Measuring baseline Ca2+ levels iin subcellular compartments using genetically engineered fluorescent indicators -- Autophagy and autophagic flux in tumor cells -- Methods to assess autophagy in situ-transmisson electron microscopy versus immunohistochemistry -- Methods to measure the enzymatic activity of P13Ks -- Luciferase-based reporter to monitor the transciptional activity of the SIRT3 promoter -- Meabolomic profiling of cultured cancer cells -- Pulsed stable isotope-resolved metaolomic studies -- Single-cell imaging for the study of oncometabolism -- Study of cellular oncometabolism via multidimensional protein identification technology -- In vivo quantitative proteomics for the study of oncometabolism -- Metabolomic profiling of neoplastic lesions in mice -- Metabolomic profiling of tumor-bearing mice -- Metabolomic studies of patient material by high-resolution magic angle spinning nuclear magnetic resonance spectroscopy -- Analysis of metabolomic profiling data acquired on GC-MS.