Cell-wide metabolic alterations associated with malignancy /

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods providing a theoretical overview on metabolic alterations of cancer cells and a series of protocols that can be employed to...

Πλήρης περιγραφή

Λεπτομέρειες βιβλιογραφικής εγγραφής
Άλλοι συγγραφείς: Galluzzi, Lorenzo, Kroemer, Guido
Μορφή: Ηλ. βιβλίο
Γλώσσα:English
Έκδοση: Amsterdam ; Boston : Academic Press, 2014.
Έκδοση:First edition.
Σειρά:Methods in enzymology ; v. 543.
Θέματα:
Διαθέσιμο Online:Full Text via HEAL-Link
Πίνακας περιεχομένων:
  • Methods to measure cytoplasmic and mitochondrial Ca2+ concentration using Ca2+ -sensitive dyes
  • Methods to measure intracellular Ca2+ fluxes with organelle-targeted aequorin-bsaed probes
  • Measuring baseline Ca2+ levels iin subcellular compartments using genetically engineered fluorescent indicators
  • Autophagy and autophagic flux in tumor cells
  • Methods to assess autophagy in situ-transmisson electron microscopy versus immunohistochemistry
  • Methods to measure the enzymatic activity of P13Ks
  • Luciferase-based reporter to monitor the transciptional activity of the SIRT3 promoter
  • Meabolomic profiling of cultured cancer cells
  • Pulsed stable isotope-resolved metaolomic studies
  • Single-cell imaging for the study of oncometabolism
  • Study of cellular oncometabolism via multidimensional protein identification technology
  • In vivo quantitative proteomics for the study of oncometabolism
  • Metabolomic profiling of neoplastic lesions in mice
  • Metabolomic profiling of tumor-bearing mice
  • Metabolomic studies of patient material by high-resolution magic angle spinning nuclear magnetic resonance spectroscopy
  • Analysis of metabolomic profiling data acquired on GC-MS.