Mechanotransduction /

• Άλλοι συγγραφείς: Engler, Adam J., Kumar, Sanjay
• Μορφή: Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: Burlington : Elsevier Science, 2014.
• Σειρά: Progress in molecular biology and translational science ; volume 126.
• Θέματα: Biomechanics.; Cells > Mechanical properties.; Cellular signal transduction.; Tissue engineering.; MEDICAL > Physiology.; SCIENCE > Life Sciences > Human Anatomy & Physiology.; Electronic books.

Progress in Molecular Biology and Translational Science provides a forum for discussion of new discoveries, approaches, and ideas in molecular biology. It contains contributions from leaders in their fields and abundant references. Volume 126 features in-depth reviews that focus on the tools require...

Πίνακας περιεχομένων:

• Front Cover; Mechanotransduction; Copyright; Contents; Contributors; Preface; Part One: Subcellular Tools for Activating and Measuring Mechanotransductive Signaling; Chapter One: The Detection and Role of Molecular Tension in Focal Adhesion Dynamics; 1. Brief Introduction to Mechanobiology; 2. Focal Adhesions in Mechanosensing; 2.1. Focal adhesion structure; 2.2. Molecular mechanisms of mechanotransduction; 3. Design and Use of Optically Based Molecular Tension Sensors; 3.1. Basics of Forster Resonance Energy Transfer (FRET); 3.2. Designs of FRET-based force-sensitive biosensors.
• 3.2.1. Extensible domain3.2.2. Rotatable domain; 3.2.3. Other designs; 3.3. Use of FRET-based tension sensors: Relative versus absolute measurements; 3.4. Critical control experiments and assumptions involved in the creation and use of FRET-based biosensors; 3.5. Conformation sensors versus tension sensors; 4. The Role of Molecular Tension in Focal Adhesion Dynamics; 5. Future Outlook; Acknowledgments; References; Chapter Two: Single-Cell Imaging of Mechanotransduction in Endothelial Cells; 1. Introduction; 2. Atherosclerosis, EC Wound Healing, and Mechanotransduction.
• 3. Signaling Molecules Involved in Mechanosensing and Mechanotransduction4. The Effect of Subcellular Structure on Mechanotransduction; 5. Focal Adhesion and FAK; 6. Tools to Monitor Signal Transduction in Live Cells; 6.1. FPs, FRET, and fluorescence lifetime imaging microscopy; 6.2. Quantitative image-based analysis for live cells; 6.3. The FRAP analysis and finite-element-based diffusion analysis; 6.4. Automatic tracking of moving cells and subcellular features; 7. Conclusion; References; Part Two: Focal Adhesions as Sensors; Chapter Three: Focal Adhesions Function as a Mechanosensor.
• 1. Introduction: The Basic Organization of Focal Adhesions2. Mechanosensitivity of Focal Adhesions; 3. Focal Adhesions and the Effects of Environmental Parameters; 4. Focal Adhesion Signals and Cell Migration; Acknowledgments; References; Chapter Four: Mechanosensation: A Basic Cellular Process; 1. Introduction; 1.1. Historical development; 1.2. Mechanosensation/-transduction; 1.3. Effects of extracellular matrix stiffness; 1.4. Stress generated by external compression/contractility; 1.5. Stress generated by cell contractility; 1.6. Biological relevance of external and internal stress.
• 2. Focal Adhesions2.1. Mechanotransduction/-signaling; 2.2. Focal adhesion proteins; 2.2.1. Vinculin; 2.2.2. Zyxin; 2.2.3. Talin; 2.2.4. Paxillin, Pyk2; 2.2.5. p130Cas; 2.2.6. Focal adhesion kinase; 2.3. Force transduction at focal adhesions; 2.4. Protein crosstalk; 2.5. Cell signaling pathways; 2.6. Translation of information gathered at focal adhesions; 2.7. Focal adherence junctions; 2.8. Measuring mechanotransduction/-sensation; 2.8.1. Flow chambers and cone and plate rheometers; 2.8.2. Magnetic and optical traps; 2.8.3. Atomic force microscopy and biomembrane force probe.