The use of CRISPR/cas9, ZFNs, TALENs in generating site specific genome alterations /

• Other Authors: Doudna, Jennifer A. (Editor), Sontheimer, Erik J. (Editor), Anders, Carolin (Contributor)
• Format: eBook
• Language: English
• Published: Amsterdam : Elsevier/Academic Press, 2014.
• Edition: First edition.
• Series: Methods in enzymology ; v. 546.
• Subjects: Genetic engineering > Laboratory manuals.; Genomics > Laboratory manuals.; Endonucleases > Laboratory manuals.; Restriction enzymes, DNA > Laboratory manuals.; Genomics.; SCIENCE > Chemistry > Industrial & Technical.; TECHNOLOGY & ENGINEERING > Chemical & Biochemical.; Endonucleases.; Genetic engineering.; Restriction enzymes, DNA.; Genetic Engineering.; CRISPR-Cas Systems.; Clustered Regularly Interspaced Short Palindromic Repeats.; DNA Restriction Enzymes.; Electronic books.; Laboratory manuals.
• Physical Description: 1 online resource (594 pages, 24 unnumbered pages of plates) : illustrations (some color).
• Bibliography: Includes bibliographical references at the end of each chapter and indexes.
• ISBN: 9780128013342; 0128013346; 9780128011850
• ISSN: 0076-6879 ; 0076-6879

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers recent research and methods development for changing the DNA sequence within the genomes of cells and organisms. Focusing on enzymes that generate double-strand breaks in DNA, the chapters describe use of molecular tools to introduce or delete genetic information at specific sites in the genomes of animal, plant and bacterial cells.