The use of CRISPR/cas9, ZFNs, TALENs in generating site specific genome alterations /

• Άλλοι συγγραφείς: Doudna, Jennifer A. (Επιμελητής έκδοσης), Sontheimer, Erik J. (Επιμελητής έκδοσης), Anders, Carolin (Συντελεστής)
• Μορφή: Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: Amsterdam : Elsevier/Academic Press, 2014.
• Έκδοση: First edition.
• Σειρά: Methods in enzymology ; v. 546.
• Θέματα: Genetic engineering > Laboratory manuals.; Genomics > Laboratory manuals.; Endonucleases > Laboratory manuals.; Restriction enzymes, DNA > Laboratory manuals.; Genomics.; SCIENCE > Chemistry > Industrial & Technical.; TECHNOLOGY & ENGINEERING > Chemical & Biochemical.; Endonucleases.; Genetic engineering.; Restriction enzymes, DNA.; Genetic Engineering.; CRISPR-Cas Systems.; Clustered Regularly Interspaced Short Palindromic Repeats.; DNA Restriction Enzymes.; Electronic books.; Laboratory manuals.

 This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers recent research and methods development for changing the DNA sequence within the genomes of cells and organisms. Focusing on enzymes that generate double-strand breaks in DNA, the chapters describe use of molecular tools to introduce or delete genetic information at specific sites in the genomes of animal, plant and bacterial cells. 

 In vitro enzymology of Cas9 --   Targeted genome editing in human cells using CRISPR/Cas nucleases and truncated guide RNAs --   Determining the specificities of TALENs, Cas9, and other genome-editing enzymes --   Genome engineering with custom recombinases --   Genome engineering in human cells --   Genome editing in human stem cells --   Tagging endogenous loci for live-cell fluorescence imaging and molecule counting using ZFNs, TALENs, and Cas9 --   Genome editing using Cas9 nickases --   Assaying break and nick-induced homologous recombination in mammalian cells using the DR-GFP reporter and Cas9 nucleases --   Adapting CRISPR/Cas9 for functional genomics screens --   The iCRISPR platform for rapid genome editing in human pluripotent stem cells --   Creating cancer translocations in human cells using Cas9 DSBs and nCas9 paired Nicks --   Genome editing for human gene therapy --   Generation of site-specific mutations in the rate genome via CRISPR/Cas9 --   CRISPR/Cas9-based genome editing in mice by single plasmid injection --   Imaging genomic elements in living cells using CRISPR/Cas9 --   Cas9-based genome editing in xenopus tropicalis --   Cas9-based genome editing in zebrafish --   Cas9-based genome editing in drosophila --   Transgene-free genome editing by germline injection of CRISPR/Cas RNA --   Cas9-based genome editing in arabidopsis and tobacco --   Multiplex engineering of industrial yeast genomes using CRISPRm --   Protein engineering of Cas9 for enhanced function.