Riboswitches as targets and tools /

• Άλλοι συγγραφείς: Burke, Donald H. (Επιμελητής έκδοσης)
• Μορφή: Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: Waltham, MA : Academic Press, an imprint of Elsevier, [2015]
• Έκδοση: First edition.
• Σειρά: Methods in enzymology ; v. 550,
• Θέματα: RNA.; RNA-protein interactions.; Messenger RNA.; Ligands (Biochemistry); Riboswitches.; SCIENCE > Life Sciences > Biochemistry.; RNA > chemistry.; Gene Expression Regulation > physiology.; Electronic books.

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in riboswitches as targets and tools and contains sections on such topics as constructing and optimizing artificial riboswi...

Πίνακας περιεχομένων:

• Design of transcription regulating riboswitches
• Ligand-dependent exponential amplification of self-replicating RNA enzymes
• Design of modular "plug-and-play" expression platforms derived from natural riboswitches for engineering novel genetically encodable RNA regulatory devices
• Integrating and amplifying signal from riboswitch biosensors
• Simple indentification of two causes of noise in an aptazyme system by monitoring cell-free transcription
• Engineering of ribosomal shunt-modulating eukaryotic ON riboswitches by using a cell-free translation system
• Live-cell imaging of mammalian RNAs with spinach2
• In vitro analysis of riboswitch-spinach aptamer fusions as metabolite-sensing fluroescent biosensors
• Using spinach aptamer to correlate mRNA and protein levels in Eschrichia coli
• Monitoring mRNA and protein levels in bulk and in model vesicle-based artificial cells
• Design, synthesis and application of spinach molecular beacons triggered by strand displacement
• Using riboswitches to regulate gene expression and define gene function in mycobacteria
• Controlling expression of genes in the unicelleular alga Chlamydomonas reinhardtii with a vitamin-repressible riboswitch
• Conditional control of gene expression by synthetic riboswitches in Streptomyces coelicolor
• Engineering of ribozyme-based aminoglycoside switches of gene expression by In Vivo genetic selection in Saccharomyces cerevisiae
• Kinetic folding design of aptazyme-regulated expression devices as riboswitches for metabolic engineering
• Riboselector: riboswitch-based synthetic selection device to expedite evolution of metabolite-producing microorgansims
• Fluorescence assays for monitoring RNA-ligand interactions and riboswitch-targeted drug discovery screening
• Monitoring ribosomal frameshifting as a platform to screen anti-riboswitch drug candidates.