Circadian rhythms and biological clocks. Volume 551, Part A /

• Άλλοι συγγραφείς: Sehgal, Amita (Επιμελητής έκδοσης)
• Μορφή: Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: Amsterdam, Netherlands : Elsevier Academic Press, 2015.
• Σειρά: Methods in enzymology ; volume 551.
• Θέματα: Circadian rhythms > Handbooks, manuals, etc.; Biological rhythms > Handbooks, manuals, etc.; SCIENCE > Life Sciences > Biochemistry.; Biological rhythms.; Circadian rhythms.; Circadian Rhythm.; Electronic books.; Handbooks and manuals.

 Circadian Rhythms and Biological Clocks Part A and Part B is an exceptional resource for anybody interested in the general area of circadian rhythms. As key elements of timekeeping are conserved in organisms across the phylogenetic tree, and our understanding of circadian biology has benefited tremendously from work done in many species, the volume provides a wide range of assays for different biological systems. Protocols are provided to assess clock function, entrainment of the clock to stimuli such as light and food, and output rhythms of behavior and physiology. This volume also delves into the impact of circadian disruption on human health. Contributions are from leaders in the field who have made major discoveries using the methods presented here. 

 Studying Circadian rhythm and sleep using genetic screens in Drosophila -- Dissecting the mechanisms of the clock in neurospora -- High-throughput and quantitative approaches for measuring circadian rhythms in cyanobacteria using bioluminescence -- Using circadian entrainment to find cryptic clocks -- Wavelet-based analysis of circadian behavioral rhythms -- Genetic analysis of Drosophila circadian behavior in seminatural conditions -- Methods to study molecular mechanisms of the neurospora circadian clock -- Detecting KaiC phosphorylation rhythms of the cyanobacterial circadian oscillator in vitro and in vivo -- The role of casein kinase I in the Drosophila circadian clock -- Purification and analysis of PERIOD protein complexes of the mammalian circadian clock -- Best practicies for fluorescence microscopy of the cyanobacterial circadian clock -- Structural and biophysical methods to analyze clock function and mechanism -- Identification of small-molecule modulators of the circadian clock -- ChIP-seq and RNA-seq methods to study circadian control of transcription in mammals -- ChIPpng away at the Drosophila clock -- Considerations for RNA-seq anlaysis of circadian rhythms -- RNA-seq profiling of small numbers of Drosophila neurons -- Analysis of circadian regulation of poly(A)-tail length -- Sample preparation of phosphoproteomic analysis of circadian time series in Arabidopsis thaliana.