Membrane proteins. Production and functional characterization /

Membrane Proteins - Production and Function Characterization a volume of Methods in Enzymology, encompasses chapters from the leading experts in the area of membrane protein biology. The chapters provide a brief overview of the topics covered and also outline step-by-step protocol. Illustrations and...

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Λεπτομέρειες βιβλιογραφικής εγγραφής
Άλλοι συγγραφείς: Shukla, Arun Kumar (Επιμελητής έκδοσης)
Μορφή: Ηλ. βιβλίο
Γλώσσα:English
Έκδοση: Amsterdam, Netherlands ; Boston, Mass. : Elsevier/ Academic Press, 2015.
Σειρά:Methods in enzymology ; v. 556.
Θέματα:
Διαθέσιμο Online:Full Text via HEAL-Link
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264 1 |a Amsterdam, Netherlands ;  |a Boston, Mass. :  |b Elsevier/ Academic Press,  |c 2015. 
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300 |a 1 online resource (xxiv, 651 pages and 14 pages of plates) :  |b illustrations (some color). 
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490 1 |a Methods in enzymology ;  |v volume 556 
505 0 |a Engineering Escherichia coli for functional expression of membrane proteins -- ACEMBLing a multiprotein transmembrane complex: the functional SecYEG-SecDF-YajC-YidC holotranslocon protein secretase/insertase -- Expression of purification of OsVDAC4 -- Membrane protein expression in Lactococcus lactis -- An unconventional anaerobic membrane protein production system based on Wolinella succinogenes -- Membrane protein expression and analysis in yeast -- Heterologous expression of G-protein-coupled receptors in yeast -- Recombinant G protein-coupled receptor expression in Saccharomyces cerevisiae for protein characterization -- Baculovirus-mediated expression of GPCRs in insect cells -- Expression of membrane proteins in the eyes of transgenic Drosophila melangaster -- Rapid method to express and purify human membrane protein using the Xenopus Oocyte system for functional and low-resolution structural anlaysis -- Expression of G protein-coupled receptors in mammalian cells -- Construction of stable mammalian cell lines for inducible expression of G protein-coupled receptors -- Rapid and facile recombinant expression of bovine rhodopsin in HEK293S GnTI- cells using a PiggyBac inducible system -- Semliki forest virus-based expression of recombinant GPCRs. 
505 0 |a Screening for lipid requirements of membrane proteins by combining cell-free expression with nanodiscs -- Liposome reconstitution and transport assay for recombinant transporters -- Lipid reconstitution and recording of recombinant ion channels -- Reconstitution of membrane proteins: a GPCR as an example -- Ion channel reporter for monitoring the activity of engineered GPCRs -- Methods for labeling skeletal muscle ion channels site-specifically with fluorophores suitable for FRET-based structural analysis -- Structural and fucntional studies of NirC from Salmonella typhimurium -- Surface plasmon resonance analysis of seven-transmembrane receptors -- Cross-linking strategies to study peptide ligand-receptor ineractions -- From recombinant expression to crystals: a step-by-step guide to GPCR crystallography -- Structure-based biophysical analysis of the interaction of rhodopsin with G protein and arrestin. 
520 |a Membrane Proteins - Production and Function Characterization a volume of Methods in Enzymology, encompasses chapters from the leading experts in the area of membrane protein biology. The chapters provide a brief overview of the topics covered and also outline step-by-step protocol. Illustrations and case example images are included wherever appropriate to help the readers understand the schematics and general experimental outlines. 
504 |a Includes bibliographical references and indexes. 
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650 0 |a Membrane proteins. 
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700 1 |a Shukla, Arun Kumar,  |e editor. 
776 0 8 |i Print version:  |t Membrane proteins.  |d Amsterdam, Netherlands ; Boston, Mass. : Elsevier/ Academic Press, 2015  |z 9780128015216  |w (OCoLC)900032183 
830 0 |a Methods in enzymology ;  |v v. 556.  |x 0076-6879 
856 4 0 |u https://www.sciencedirect.com/science/bookseries/00766879/556  |z Full Text via HEAL-Link