Membrane proteins. Production and functional characterization /
Membrane Proteins - Production and Function Characterization a volume of Methods in Enzymology, encompasses chapters from the leading experts in the area of membrane protein biology. The chapters provide a brief overview of the topics covered and also outline step-by-step protocol. Illustrations and...
| Άλλοι συγγραφείς: | |
|---|---|
| Μορφή: | Ηλ. βιβλίο |
| Γλώσσα: | English |
| Έκδοση: |
Amsterdam, Netherlands ; Boston, Mass. :
Elsevier/ Academic Press,
2015.
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| Σειρά: | Methods in enzymology ;
v. 556. |
| Θέματα: | |
| Διαθέσιμο Online: | Full Text via HEAL-Link |
Πίνακας περιεχομένων:
- Engineering Escherichia coli for functional expression of membrane proteins
- ACEMBLing a multiprotein transmembrane complex: the functional SecYEG-SecDF-YajC-YidC holotranslocon protein secretase/insertase
- Expression of purification of OsVDAC4
- Membrane protein expression in Lactococcus lactis
- An unconventional anaerobic membrane protein production system based on Wolinella succinogenes
- Membrane protein expression and analysis in yeast
- Heterologous expression of G-protein-coupled receptors in yeast
- Recombinant G protein-coupled receptor expression in Saccharomyces cerevisiae for protein characterization
- Baculovirus-mediated expression of GPCRs in insect cells
- Expression of membrane proteins in the eyes of transgenic Drosophila melangaster
- Rapid method to express and purify human membrane protein using the Xenopus Oocyte system for functional and low-resolution structural anlaysis
- Expression of G protein-coupled receptors in mammalian cells
- Construction of stable mammalian cell lines for inducible expression of G protein-coupled receptors
- Rapid and facile recombinant expression of bovine rhodopsin in HEK293S GnTI- cells using a PiggyBac inducible system
- Semliki forest virus-based expression of recombinant GPCRs.
- Screening for lipid requirements of membrane proteins by combining cell-free expression with nanodiscs
- Liposome reconstitution and transport assay for recombinant transporters
- Lipid reconstitution and recording of recombinant ion channels
- Reconstitution of membrane proteins: a GPCR as an example
- Ion channel reporter for monitoring the activity of engineered GPCRs
- Methods for labeling skeletal muscle ion channels site-specifically with fluorophores suitable for FRET-based structural analysis
- Structural and fucntional studies of NirC from Salmonella typhimurium
- Surface plasmon resonance analysis of seven-transmembrane receptors
- Cross-linking strategies to study peptide ligand-receptor ineractions
- From recombinant expression to crystals: a step-by-step guide to GPCR crystallography
- Structure-based biophysical analysis of the interaction of rhodopsin with G protein and arrestin.
