|
|
|
|
| LEADER |
05456nam a2200517 4500 |
| 001 |
ocn909368064 |
| 003 |
OCoLC |
| 005 |
20180501122025.0 |
| 006 |
m o d |
| 007 |
cr cnu|||unuuu |
| 008 |
150519s2015 ne a ob 001 0 eng d |
| 040 |
|
|
|a N$T
|b eng
|e rda
|e pn
|c N$T
|d IDEBK
|d N$T
|d OPELS
|d CUS
|d N$T
|d YDXCP
|d EBLCP
|d CDX
|d OCLCF
|d OCLCQ
|d GrThAP
|
| 019 |
|
|
|a 910446828
|a 919400033
|
| 020 |
|
|
|a 9780128026397
|q (electronic bk.)
|
| 020 |
|
|
|a 0128026391
|q (electronic bk.)
|
| 020 |
|
|
|a 9780128024508
|
| 035 |
|
|
|a (OCoLC)909368064
|z (OCoLC)910446828
|z (OCoLC)919400033
|
| 050 |
|
4 |
|a QH585.2
|b .B85
|
| 072 |
|
7 |
|a SCI
|x 056000
|2 bisacsh
|
| 082 |
0 |
4 |
|a 571.638
|2 23
|
| 049 |
|
|
|a TEFA
|
| 245 |
0 |
0 |
|a Building a cell from its component parts /
|c edited by Jennifer Ross, Wallace F. Marshall.
|
| 250 |
|
|
|a First edition.
|
| 264 |
|
1 |
|a Amsterdam :
|b Academic Press,
|c 2015.
|
| 300 |
|
|
|a 1 online resource :
|b color illustrations.
|
| 336 |
|
|
|a text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a computer
|b c
|2 rdamedia
|
| 338 |
|
|
|a online resource
|b cr
|2 rdacarrier
|
| 490 |
1 |
|
|a Methods in cell biology,
|x 0091-679X ;
|v volume 128
|
| 588 |
0 |
|
|a Online resource; title from PDF title page (EBSCO, viewed May 28, 2015).
|
| 504 |
|
|
|a Includes bibliographical references and index.
|
| 505 |
0 |
|
|a Front Cover; Methods in Cell Biology; Series Editors; Methods in Cell Biology; Copyright; Contents; Contributors; Preface; 1. In vitro systems for the study of microtubule-based cell polarity in fission yeast; Introduction; 1. Rationale; 2. Materials; 2.1 Materials for Method 1: Elongated Glass Wells with TiO2 Overhang; 2.1.1 Microfabrication; 2.1.2 Surface functionalization; 2.1.3 Assay; 2.2 Materials for Method 2: Elongated Water in Oil Emulsion Droplets; 2.2.1 Microfluidic chip; 3. Methods; 3.1 Method 1: Elongated Glass Wells with TiO2 Overhang; 3.1.1 Design.
|
| 505 |
8 |
|
|a 3.1.2 Deposition of chromium and titanium oxide by electron-beam evaporation3.1.3 Postannealing of titanium oxide; 3.1.4 UV-lithography with S1813; 3.1.5 Reactive ion etching; 3.1.6 Wet etching with KOH; 3.1.7 Surface functionalization; 3.1.8 Assays; 3.2 Method 2: Elongated Water in Oil Emulsion Droplets; 3.2.1 Microfluidic design; 3.2.2 Masks for microfluidic chip fabrication; 3.2.3 SU-8 negative mould; 3.2.4 PDMS chip (Figure 5); 3.2.5 Microfluidic setup and droplet formation; 3.2.6 Lipids composition; 3.2.7 Assays; Discussion and Perspectives; Acknowledgments; References.
|
| 505 |
8 |
|
|a 2. Microtubules, MAPs, and motor patternsIntroduction; 1. Methods; 2. Simple Filament-gliding Assay with Kinesin-1 and Microtubules; 2.1 Flow Chambers; 2.1.1 Materials; 2.1.2 Chamber construction; 2.1.3 Notes; 2.2 Gliding Assay Reagents and Buffers; 2.3 Experimental Details; 2.4 Analysis and Notes; 2.4.1 Kymographs; 2.4.2 Filament end tracking; 2.4.3 Notes; 3. Gliding Assay with MAP65 Cross-linkers to Visualize Dynamic Cross-linking; 3.1 MAP65 Purification; 3.1.1 Preparation reagents and buffers; 3.1.2 Protein purification; 3.1.2.1 Day 1; 3.1.2.2 Day 2; 3.1.2.3 Day 3.
|
| 505 |
8 |
|
|a 3.2 Gliding Assay Buffers3.3 Experimental Details; 3.4 Analysis and Notes; 4. Cell-like Patterns from Gliding Prebundled Microtubule Filaments; 4.1 Reagents and Buffers; 4.2 Prebundling Microtubules; 4.3 Experimental Details; Discussion and Summary; Acknowledgments; References; 3. Self-organization of motors and microtubules in lipid-monolayered droplets; Introduction; 1. Materials; 1.1 Equipment; 1.1.1 Glass surface treatment and Polydimethylsiloxane (PDMS) chamber preparation; 1.1.2 Lipid-oil mixture preparation; 1.1.3 Buffer droplet in oil production; 1.1.4 Confocal fluorescence microscopy.
|
| 505 |
8 |
|
|a 1.2 Reagents1.2.1 For glass surface treatment and PDMS chamber preparation; 1.2.2 For lipid-oil mixture preparation and buffer droplet in oil production; 1.3 Buffers and Solutions; 1.4 Proteins Used in the Assays; 2. Methods; 2.1 Glass Surface Treatment and PDMS Chamber Preparation; 2.1.1 Cleaning and hydrophobic surface treatment; 2.1.2 PDMS chambers; 2.2 Lipid-Oil Mixture Preparation; 2.3 Buffer Droplet in Oil Production; 2.3.1 Droplet solution preparation; 2.3.2 Resuspension of droplets in oil; 2.4 Confocal Fluorescence Microscopy; Results and Discussion; Acknowledgments; References.
|
| 520 |
|
|
|a This new volume of Methods in Cell Biology looks at building a cell from its component parts. Chapters cover such topics as engineering motor scaffolds, artificial cytoskeletons, interconnected droplet networks and artificial cells; building cytoskeletal systems and artificial actin cortex on pillar arrays; reconstituting membrane fission, actin bundles at membranes, actin cortex in droplets, dynein-dynactin mediated cargo transport, MinD systems, protein-lipid machines that transform membranes, protein interactions and signaling on artificial lipid bilayers. With cutting-edge material, this c.
|
| 650 |
|
0 |
|a Cell culture.
|
| 650 |
|
7 |
|a SCIENCE
|x Life Sciences
|x Anatomy & Physiology.
|2 bisacsh
|
| 650 |
|
7 |
|a Cell culture.
|2 fast
|0 (OCoLC)fst00850172
|
| 655 |
|
4 |
|a Electronic books.
|
| 700 |
1 |
|
|a Ross, Jennifer,
|e editor.
|
| 700 |
1 |
|
|a Marshall, Wallace F.,
|d 1968-
|e editor.
|
| 776 |
0 |
8 |
|i Print version:
|a Ross, Jennifer.
|t Building a Cell from Its Component Parts.
|d Burlington : Elsevier Science, ©2015
|z 9780128024508
|
| 830 |
|
0 |
|a Methods in cell biology ;
|v v. 128.
|
| 856 |
4 |
0 |
|u https://www.sciencedirect.com/science/bookseries/0091679X/128
|z Full Text via HEAL-Link
|
