Laboratory methods in enzymology. Protein, Part D /
In this volume we have brought together a number of core protocols concentrating on Protein, carefully written and edited by experts.
| Άλλοι συγγραφείς: | |
|---|---|
| Μορφή: | Ηλ. βιβλίο |
| Γλώσσα: | English |
| Έκδοση: |
London :
Elsevier Academic Press,
2015.
©2015 |
| Έκδοση: | First edition. |
| Σειρά: | Methods in enzymology ;
v. 559. |
| Θέματα: | |
| Διαθέσιμο Online: | Full Text via HEAL-Link |
| LEADER | 05553nam a2200577 4500 | ||
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| 245 | 0 | 0 | |a Laboratory methods in enzymology. |p Protein, |n Part D / |c edited by Jon R. Lorsch. |
| 250 | |a First edition. | ||
| 260 | |a London : |b Elsevier Academic Press, |c 2015. | ||
| 264 | 4 | |c ©2015 | |
| 300 | |a 1 online resource (xviii, 470 pages) : |b color illustrations. | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 490 | 1 | |a Methods in Enzymology, |x 0076-6879 ; |v volume 559 | |
| 504 | |a Includes bibliographical references and index. | ||
| 505 | 0 | 0 | |t Purification of His-tagged proteins -- |t Affinity purification of a recombinant protein expressed as a fusion with the maltose-binding protein (MBP) tag -- |t Immunoaffinity purification of proteins -- |t Affinity purification of protein complexes using TAP tags -- |t Strep-tagged protein purification -- |t Proteoloytic affinity tag cleavage -- |t Affinity pull-down of proteins using anti-FLAG M2 agarose beads -- |t Protein affinity prufication using intein-chitin binding protein tags -- |t Purification of GST-tagged proteins. |
| 505 | 0 | |a Front Cover; Laboratory Methods in Enzymology: Protein Part D; Copyright; Contents; Contributors; Preface; Chapter One: Purification of His-Tagged Proteins; 1. Theory; 2. Equipment; 3. Materials; 3.1. Solutions & buffers; 4. Protocol; 4.1. Preparation; 4.2. Duration; 5. Protocol A: Purification of His-tagged Proteins Under Native Conditions; 5.1. Tip; 6. Step 1A Preparation of a Cleared E. coli Lysate Under Native Conditions; 6.1. Overview; 6.2. Duration; 6.3. Tip; 6.4. Tip; 6.5. Tip; 6.6. Tip; 6.7. Tip; 7. Step 2A Batch Purification of His-tagged Proteins from E. coli Under Native Conditions. | |
| 505 | 8 | |a 7.1. Overview7.2. Duration; 7.3. Tip; 7.4. Tip; 7.5. Tip; 7.6. Tip; 8. Step 1B Preparation of a Cleared E. coli Lysate Under Denaturing Conditions; 8.1. Overview; 8.2. Duration; 8.3. Tip; 8.4. Tip; 8.5. Tip; 8.6. Tip; 8.7. Tip; 9. Step 2B Batch Purification of His-tagged Proteins from E. coli Under Denaturing Conditions; 9.1. Overview; 9.2. Duration; 9.3. Tip; 9.4. Tip; 9.5. Tip; 9.6. Tip; References; Referenced Literature; Source References; Referenced Protocols in Methods Navigator. | |
| 505 | 8 | |a Chapter Two: Affinity Purification of a Recombinant Protein Expressed as a Fusion with the Maltose-Binding Protein (MBP) Tag1. Theory; 2. Equipment; 3. Materials; 3.1. Solutions & buffers; 4. Protocol; 4.1. Duration; 4.2. Preparation; 5. Step 1 Equilibration of the Column; 5.1. Overview; 5.2. Duration; 5.3. Tip; 5.4. Tip; 6. Step 2 Binding of the Protein Sample; 6.1. Overview; 6.2. Duration; 6.3. Tip; 6.4. Tip; 7. Step 3 Removal of Unbound Proteins; 7.1. Overview; 7.2. Duration; 8. Step 4 Elution of the Bound Protein; 8.1. Overview; 8.2. Duration; 8.3. Tip; 8.4. Tip; 8.5. Tip; 8.6. Tip. | |
| 505 | 8 | |a 8.7. Tip8.8. Tip; References; Referenced Literature; Referenced Protocols in Methods Navigator; Chapter Three: Immunoaffinity Purification of Proteins; 1. Theory; 2. Equipment; 3. Materials; 3.1. Solutions & buffers; 4. Protocol; 4.1. Duration; 4.2. Preparation; 4.3. Tip; 4.4. Tip; 5. Step 1 Equilibrate Column; 5.1. Overview; 5.2. Duration; 5.3. Tip; 5.4. Tip; 6. Step 2 Loading the Column; 6.1. Overview; 6.2. Duration; 6.3. Tip; 7. Step 3 Washing the Column; 7.1. Overview; 7.2. Duration; 7.3. Tip; 8. Step 4 Elution of the Protein from the Column; 8.1. Overview; 8.2. Duration; 8.3. Tip. | |
| 505 | 8 | |a 8.4. Tip8.5. Tip; 8.6. Tip; 9. Step 5 Column Regeneration and Storage; 9.1. Overview; 9.2. Duration; 9.3. Tip; References; Referenced Protocols in Methods Navigator; Chapter Four: Affinity Purification of Protein Complexes Using TAP Tags; 1. Theory; 2. Equipment; 3. Materials; 3.1. Solutions & buffers; 4. Protocol; 4.1. Preparation; 4.2. Duration; 5. Step 1 Preparation of Protein Lysates; 5.1. Overview; 5.2. Duration; 5.3. Tip; 5.4. Tip; 5.5. Tip; 6. Step 2 Binding of the Protein A Tag to IgG-Sepharose; 6.1. Overview; 6.2. Duration; 6.3. Tip; 6.4. Tip; 6.5. Tip; 6.6. Tip 7 Step 3 TEV Protease Cleave. | |
| 588 | 0 | |a Print version record. | |
| 520 | |a In this volume we have brought together a number of core protocols concentrating on Protein, carefully written and edited by experts. | ||
| 650 | 0 | |a Proteins |x Analysis. | |
| 650 | 7 | |a SCIENCE |x Life Sciences |x Biochemistry. |2 bisacsh | |
| 650 | 7 | |a Proteins |x Analysis. |2 fast |0 (OCoLC)fst01079714 | |
| 650 | 7 | |a Proteins |x Purification. |2 fast |0 (OCoLC)fst01079750 | |
| 655 | 4 | |a Electronic books. | |
| 655 | 7 | |a Electronic books. |2 lcgft | |
| 700 | 1 | |a Lorsch, Jon, |e editor. | |
| 776 | 0 | 8 | |i Print version: |a Lorsch, Jon. |t Laboratory Methods in Enzymology: Protein Part D. |d Burlington : Elsevier Science, ©2015 |z 9780128002797 |
| 830 | 0 | |a Methods in enzymology ; |v v. 559. |x 0076-6879 | |
| 856 | 4 | 0 | |u https://www.sciencedirect.com/science/bookseries/00766879/559 |z Full Text via HEAL-Link |
