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05475cam a2200541Ii 4500 |
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ocn920673617 |
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OCoLC |
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20180501122027.0 |
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m o d |
| 007 |
cr cnu|||unuuu |
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150910s2015 ne a ob 001 0 eng d |
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|a N$T
|b eng
|e rda
|e pn
|c N$T
|d N$T
|d OPELS
|d YDXCP
|d CUS
|d IDEBK
|d UIU
|d CDX
|d OCLCF
|d EBLCP
|d NLGGC
|d OCLCQ
|d CASUM
|d OCLCO
|d OCLCA
|d GrThAP
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| 019 |
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|a 929521402
|a 932328782
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|a 9780128028711
|q electronic bk.
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|a 0128028718
|q electronic bk.
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| 020 |
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|a 9780128028292
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|a (OCoLC)920673617
|z (OCoLC)929521402
|z (OCoLC)932328782
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| 050 |
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4 |
|a QH603.E63
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| 072 |
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7 |
|a SCI
|x 056000
|2 bisacsh
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0 |
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|a 571.655
|2 23
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| 049 |
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|a TEFA
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| 245 |
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|a Sorting and recycling endosomes /
|c edited by Wei Guo.
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| 250 |
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|a First edition.
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| 264 |
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1 |
|a Amsterdam :
|b Academic Press is an imprint of Elsevier,
|c 2015.
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| 300 |
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|a 1 online resource (xix, 396 pages) :
|b illustrations (some color).
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in cell biology ;
|v v. 130
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| 504 |
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|a Includes bibliographical references and index.
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| 588 |
0 |
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|a Online resource; title from PDF title page (EBSCO, viewed September 11, 2015).
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| 520 |
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|a Sorting and Recycling Endosomes provides the latest information on endosomes, the receiving compartment for endocytosed cargos, and the donor compartment and sorting station for cargos designated to lysosomes, Golgi, or plasma membrane. In recent years, the importance of endosomes as a sorting and recycling compartment has become increasingly appreciated. As such, scientists from various fields of cell biology, membrane traffic, and beyond, see the needs to communicate and learn about the methods used to investigate the dynamics and functions of endosomes. This book brings together specialists from the field who contribute their expertise on a broad range of biomedical topics that will provide ideal reading for researchers interested in endosomal sorting and recycling. This volume covers the approaches necessary to study the key components that mediate the generation and transport of membrane-bounded carriers from the endosomes, and how membrane trafficking machinery is coordinated with cytoskeletons during these processes. In addition to studies carried out in mammalian cells, other model systems such as worm and yeast are also included.
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| 505 |
0 |
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|a Front Cover; Methods in Cell Biology: Sorting and Recycling Endosomes; Series Editors; Methods in Cell Biology: Sorting and Recycling Endosomes; Copyright; Contents; Contributors; Preface; 1. Dynamic imaging of the recycling endosomal network in macrophages; Introduction; Introduction; 1. Methods; 1.1 Cell Culture and Transfection; 1.2 Materials and Instruments; 1.2.1 Reagents; 1.2.2 Instruments; 1.3 Live Cell Imaging; 2. Experimental Strategies; 2.1 Strategy 1: RE Subcompartments Defined by Rabs; 2.1.1 Background and objective; 2.1.2 Flow of the experiment; 2.1.3 Results
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| 505 |
8 |
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|a 2.2 Strategy 2: Cargo Movement through RE2.2.1 Background and Objective; 2.2.2 Flow of the experiments; Experiment 1: Recycling endosome component trafficking pathways; Experiment 1: Recycling endosome component trafficking pathways; Experiment 2: Rates of Transferrin Trafficking from the Cell Surface; Experiment 2: Rates of Transferrin Trafficking from the Cell Surface; 2.2.3 Experimental considerations; 2.2.4 Results; 2.3 Strategy 3: Delivery of RE to Phagosomes; 2.3.1 Background and objective; 2.3.2 Flow of the experiment; 2.3.3 Experimental considerations; 2.3.4 Results; Summary; Summary
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| 505 |
8 |
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|a 2.1.2 Data interpretation2.2 Analyzing Actin or Microtubule Dynamics during Cytokinesis; 2.3 Cytokinesis and Apical Lumen Formation in 3D Cultures; 2.3.1 Procedure; Summary; Acknowledgments; References; 3. Expression and localization of exocytic and recycling Rabs from Magnaporthe oryzae in mammalian cells; Introduction; Introduction; 1. Materials and Equipment; 1.1 Reagents for Cloning and Plasmid Construction; 1.2 Reagents for Protein Expression and Analysis; 1.3 Materials and Reagents for Confocal Fluorescence Microscopy; 1.4 Equipment; 2. Methods
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| 505 |
8 |
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|a 2.1 Cloning of MoRab11, MoRab8, and MoRab12.1.1 Extraction of total RNA from the M. oryzae strain Guy11; 2.1.2 Amplifying the cDNAs of MoRab11, MoRab8, and MoRab1 by RT-PCR and cloning into the bidirectional expression vector pBI-Tet ...; 2.2 Expression and Intracellular Localization of MoRab11, MoRab8, and MoRab1; 2.2.1 Transfection of BHK-21 cells; 2.2.2 Immunoblot analysis of protein expression; 2.2.3 Localization by confocal fluorescence microscopy; Summary; Acknowledgments; References; 4. Methods to analyze novel liaisons between endosomes and centrosomes
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| 650 |
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0 |
|a Endosomes.
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| 650 |
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0 |
|a Cell interaction.
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| 650 |
|
7 |
|a SCIENCE / Life Sciences / Anatomy & Physiology
|2 bisacsh
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| 650 |
|
7 |
|a Cell interaction.
|2 fast
|0 (OCoLC)fst00850189
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| 650 |
|
7 |
|a Endosomes.
|2 fast
|0 (OCoLC)fst01740259
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| 650 |
1 |
2 |
|a Endosomes.
|0 (DNLM)D011992
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| 655 |
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4 |
|a Electronic books.
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| 655 |
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0 |
|a Electronic books.
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| 700 |
1 |
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|a Guo, Wei,
|e editor.
|
| 776 |
0 |
8 |
|i Print version:
|a Guo, Wei
|t Sorting and Recycling Endosomes
|d : Elsevier Science,c2015
|z 9780128028292
|
| 830 |
|
0 |
|a Methods in cell biology ;
|v v. 130.
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| 856 |
4 |
0 |
|u https://www.sciencedirect.com/science/bookseries/0091679X/130
|z Full Text via HEAL-Link
|
