Sorting and recycling endosomes /

• Άλλοι συγγραφείς: Guo, Wei (Επιμελητής έκδοσης)
• Μορφή: Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: Amsterdam : Academic Press is an imprint of Elsevier, 2015.
• Έκδοση: First edition.
• Σειρά: Methods in cell biology ; v. 130.
• Θέματα: Endosomes.; Cell interaction.; SCIENCE / Life Sciences / Anatomy & Physiology; Electronic books.

Sorting and Recycling Endosomes provides the latest information on endosomes, the receiving compartment for endocytosed cargos, and the donor compartment and sorting station for cargos designated to lysosomes, Golgi, or plasma membrane. In recent years, the importance of endosomes as a sorting and r...

Πίνακας περιεχομένων:

• Front Cover; Methods in Cell Biology: Sorting and Recycling Endosomes; Series Editors; Methods in Cell Biology: Sorting and Recycling Endosomes; Copyright; Contents; Contributors; Preface; 1. Dynamic imaging of the recycling endosomal network in macrophages; Introduction; Introduction; 1. Methods; 1.1 Cell Culture and Transfection; 1.2 Materials and Instruments; 1.2.1 Reagents; 1.2.2 Instruments; 1.3 Live Cell Imaging; 2. Experimental Strategies; 2.1 Strategy 1: RE Subcompartments Defined by Rabs; 2.1.1 Background and objective; 2.1.2 Flow of the experiment; 2.1.3 Results
• 2.2 Strategy 2: Cargo Movement through RE2.2.1 Background and Objective; 2.2.2 Flow of the experiments; Experiment 1: Recycling endosome component trafficking pathways; Experiment 1: Recycling endosome component trafficking pathways; Experiment 2: Rates of Transferrin Trafficking from the Cell Surface; Experiment 2: Rates of Transferrin Trafficking from the Cell Surface; 2.2.3 Experimental considerations; 2.2.4 Results; 2.3 Strategy 3: Delivery of RE to Phagosomes; 2.3.1 Background and objective; 2.3.2 Flow of the experiment; 2.3.3 Experimental considerations; 2.3.4 Results; Summary; Summary
• 2.1.2 Data interpretation2.2 Analyzing Actin or Microtubule Dynamics during Cytokinesis; 2.3 Cytokinesis and Apical Lumen Formation in 3D Cultures; 2.3.1 Procedure; Summary; Acknowledgments; References; 3. Expression and localization of exocytic and recycling Rabs from Magnaporthe oryzae in mammalian cells; Introduction; Introduction; 1. Materials and Equipment; 1.1 Reagents for Cloning and Plasmid Construction; 1.2 Reagents for Protein Expression and Analysis; 1.3 Materials and Reagents for Confocal Fluorescence Microscopy; 1.4 Equipment; 2. Methods
• 2.1 Cloning of MoRab11, MoRab8, and MoRab12.1.1 Extraction of total RNA from the M. oryzae strain Guy11; 2.1.2 Amplifying the cDNAs of MoRab11, MoRab8, and MoRab1 by RT-PCR and cloning into the bidirectional expression vector pBI-Tet ...; 2.2 Expression and Intracellular Localization of MoRab11, MoRab8, and MoRab1; 2.2.1 Transfection of BHK-21 cells; 2.2.2 Immunoblot analysis of protein expression; 2.2.3 Localization by confocal fluorescence microscopy; Summary; Acknowledgments; References; 4. Methods to analyze novel liaisons between endosomes and centrosomes