Analytical ultracentrifugation /
Analytical Ultracentrifugation, the latest volume in Methods in Enzymology, focuses on analytical ultracentrifugation. The scope of this technique has greatly expanded in recent years due to advances in instrumentation, algorithms and software. This volume describes the latest innovations in the fie...
| Άλλοι συγγραφείς: | |
|---|---|
| Μορφή: | Ηλ. βιβλίο |
| Γλώσσα: | English |
| Έκδοση: |
London, UK :
Academic Press,
2015.
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| Έκδοση: | First edition. |
| Σειρά: | Methods in enzymology ;
v. 562. |
| Θέματα: | |
| Διαθέσιμο Online: | Full Text via HEAL-Link |
Πίνακας περιεχομένων:
- Title page; Table of Contents; Copyright; Contributors; Preface; Chapter One: Next-Generation AUC Adds a Spectral Dimension: Development of Multiwavelength Detectors for the Analytical Ultracentrifuge; Abstract; 1 Introduction; 2 Development of MWL Absorbance Detectors; 3 Hardware and Control System of Second-Generation MWL Detectors; 4 MWL Data From AUC; 5 Data Visualization and Experimental Results; 6 Discussion; 7 Summary and General Discussion of the Impacts of MWL AUC Data in the Field of Biopolymers; Acknowledgments; Supplemental Information
- Chapter Two: Next-Generation AUC: Analysis of Multiwavelength Analytical Ultracentrifugation DataAbstract; 1 Introduction; 2 Computational Treatment of Multiwavelength Data; 3 Applications; 4 Discussion; 5 Conclusion; Acknowledgments; Chapter Three: Sedimentation Velocity: A Classical Perspective; Abstract; 1 Early History; 2 Irreversible Thermodynamics; 3 Sedimentation Velocity; 4 Balanced Forces in Diffusion; 5 Balanced Forces in Sedimentation; 6 Derivation of the Lamm Equation; 7 Heterogeneous Paucidisperse Systems; 8 Nonideal Systems; 9 Interacting Systems
- 10 Solutions of the Lamm Equation11 Equipment and Optical Systems; 12 Sedimentation Velocity Experimental Design; Acknowledgments; Chapter Four: Hydrodynamic Modeling and Its Application in AUC; Abstract; 1 Introduction; 2 AUC and Hydrodynamics; 3 The Hydration Issue; 4 Segmental Flexibility and Intrinsically Disordered Structures; 5 Available Programs for Rigid Body HM; 6 Comparing the Performance of Various Methods; 7 A Step-by-Step Guide to Performing Correct HM Using Atomic Coordinates; 8 Selected Literature Examples; 9 Concluding Remarks
- Chapter Five: Calculations and Publication-Quality Illustrations for Analytical Ultracentrifugation DataAbstract; 1 Introduction; 2 General Features of GUSSI; 3 Utility Functions of GUSSI; 4 Summary; Acknowledgments; Chapter Six: Sedimentation Equilibrium Analysis of ClpB Self-Association in Diluted and Crowded Solutions; Abstract; 1 Introduction; 2 Experimental Approaches and Analysis; 3 Summary of Experimental Results; 4 ClpB Association Equilibrium and Disaggregase Activity; 5 Concluding Remarks; Acknowledgments; Chapter Seven: Analysis of Linked Equilibria; Abstract; 1 Introduction
- 2 Determination of Ln,0 for an Assembling System3 Global Fitting of Sedimentation Velocity Data as a Function of Protein Concentration Kinetic Considerations; 4 Global Analysis Using the 1-2-4-6 Model with Rapid Dissociating Oligomers; 5 Global Analysis Using the 1-2-4-6 Model with Slow Dissociation of Oligomers; 6 Global Analysis with Rate Constants in the Detectable Range; 7 Conclusions; Chapter Eight: Elucidating Complicated Assembling Systems in Biology Using Size-and-Shape Analysis of Sedimentation Velocity Data; Abstract; 1 Techniques for Studying Macromolecular Mixtures
