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03530nam a2200529 4500 |
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ocn934769600 |
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20180501122028.0 |
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160115s2016 maua ob 001 0 eng d |
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|a IDEBK
|b eng
|e rda
|e pn
|c IDEBK
|d N$T
|d BUF
|d YDXCP
|d N$T
|d UIU
|d OCLCF
|d OCLCQ
|d CUS
|d OCLCO
|d EBLCP
|d CDX
|d OCLCQ
|d KUM
|d CASUM
|d GrThAP
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|c (S
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|a 935919762
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|a 9780128029077
|q (electronic bk.)
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|a 0128029072
|q (electronic bk.)
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|a 9780128029060
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|a 0128029064
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|a (OCoLC)934769600
|z (OCoLC)935919762
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|a QC291
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|a SCI
|x 065000
|2 bisacsh
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|a 536.6
|2 23
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|a TEFA
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|a Calorimetry /
|c edited by Andrew L. Feig.
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|a First edition.
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|a Cambridge, MA :
|b Academic Press,
|c 2016.
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|c Ã2016
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|a 1 online resource (xix, 493 pages) :
|b illustrations (chiefly color).
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|a text
|b txt
|2 rdacontent
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|a computer
|b c
|2 rdamedia
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|a online resource
|b cr
|2 rdacarrier
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|a Methods in enzymology,
|x 0076-6879 ;
|v volume 567
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|a Includes bibliographical references and indexes.
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|a Print version record.
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|6 880-01
|a 6. Additional Considerations with Metal Ions6.1. Comparison with Other Methods; 6.2. Sources of Error; 6.3. Tips; 7. Conclusion; Acknowledgments; References; Chapter Two: Assessing Coupled Protein Folding and Binding Through Temperature-Dependent Isothermal Titration Calorimetry; 1. Introduction; 2. Sample Preparation; 2.1. Plasmid Construction; 2.2. Purification of FCP1 and Rap74; 3. Special Considerations for ITC of Intrinsically Disordered Proteins; 3.1. Experimental Design; 3.2. Calorimetric Parameters; 4. Data Fitting and Analysis; 4.1. Standard Data Fitting in Origin
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|a 4.2. Data Fitting in MATLAB4.3. Global Analysis of Temperature-Dependent Data Sets; 5. Summary; Acknowledgments; References; Chapter Three: Fragment-Based Screening for Enzyme Inhibitors Using Calorimetry; 1. Introduction; 1.1. Nanocalorimeter Arrays; 1.2. Fragment-Based Lead Discovery; 2. Required Materials; 2.1. Equipment; 2.2. Reagents; 2.3. Software; 3. Instrumentation; 4. Fragment Library Selection; 4.1. Materials Needed; 4.2. Software Needed; 4.3. Protocol to Prepare of Fragment Library for Screening; 5. Calorimetric Enzyme Assay: Nanocalorimeter
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|a 5.1. Sample Considerations and Preparation5.2. Data Analysis; 5.3. Protocol; 6. Calorimetric Enzyme Assay: Commercial Microcalorimeters; 6.1. Required Materials; 6.2. Cleaning Before and After Runs; 6.3. Protocol 2: Microcalorimeter; 6.4. Data Processing and Analysis; 7. Summary; Acknowledgments; References; Chapter Four: Measuring Multivalent Binding Interactions by Isothermal Titration Calorimetry; 1. Introduction; 2. Determining the Thermodynamics of Lectin Binding to Multivalent Synthetic Analogs; 2.1. Methods; 2.1.1. Isothermal Titration Calorimetry
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|a Calorimetry.
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|a SCIENCE
|x Mechanics
|x Thermodynamics.
|2 bisacsh
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|a Calorimetry.
|2 fast
|0 (OCoLC)fst00844526
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|a Calorimetry.
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|a Electronic books.
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|a Electronic books.
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|a Feig, Andrew L.,
|e editor.
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776 |
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|i Print version:
|a Feig, Andrew
|t Calorimetry
|d : Elsevier Science,c2016
|z 9780128029060
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830 |
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|a Methods in enzymology ;
|v v. 567.
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856 |
4 |
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|u https://www.sciencedirect.com/science/bookseries/00766879/567
|z Full Text via HEAL-Link
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