Calorimetry /

Λεπτομέρειες βιβλιογραφικής εγγραφής
Άλλοι συγγραφείς: Feig, Andrew L. (Επιμελητής έκδοσης)
Μορφή: Ηλ. βιβλίο
Γλώσσα:English
Έκδοση: Cambridge, MA : Academic Press, 2016.
Έκδοση:First edition.
Σειρά:Methods in enzymology ; v. 567.
Θέματα:
Διαθέσιμο Online:Full Text via HEAL-Link
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245 0 0 |a Calorimetry /  |c edited by Andrew L. Feig. 
250 |a First edition. 
264 1 |a Cambridge, MA :  |b Academic Press,  |c 2016. 
264 4 |c Ã2016 
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490 1 |a Methods in enzymology,  |x 0076-6879 ;  |v volume 567 
504 |a Includes bibliographical references and indexes. 
588 0 |a Print version record. 
505 8 |6 880-01  |a 6. Additional Considerations with Metal Ions6.1. Comparison with Other Methods; 6.2. Sources of Error; 6.3. Tips; 7. Conclusion; Acknowledgments; References; Chapter Two: Assessing Coupled Protein Folding and Binding Through Temperature-Dependent Isothermal Titration Calorimetry; 1. Introduction; 2. Sample Preparation; 2.1. Plasmid Construction; 2.2. Purification of FCP1 and Rap74; 3. Special Considerations for ITC of Intrinsically Disordered Proteins; 3.1. Experimental Design; 3.2. Calorimetric Parameters; 4. Data Fitting and Analysis; 4.1. Standard Data Fitting in Origin 
505 8 |a 4.2. Data Fitting in MATLAB4.3. Global Analysis of Temperature-Dependent Data Sets; 5. Summary; Acknowledgments; References; Chapter Three: Fragment-Based Screening for Enzyme Inhibitors Using Calorimetry; 1. Introduction; 1.1. Nanocalorimeter Arrays; 1.2. Fragment-Based Lead Discovery; 2. Required Materials; 2.1. Equipment; 2.2. Reagents; 2.3. Software; 3. Instrumentation; 4. Fragment Library Selection; 4.1. Materials Needed; 4.2. Software Needed; 4.3. Protocol to Prepare of Fragment Library for Screening; 5. Calorimetric Enzyme Assay: Nanocalorimeter 
505 8 |a 5.1. Sample Considerations and Preparation5.2. Data Analysis; 5.3. Protocol; 6. Calorimetric Enzyme Assay: Commercial Microcalorimeters; 6.1. Required Materials; 6.2. Cleaning Before and After Runs; 6.3. Protocol 2: Microcalorimeter; 6.4. Data Processing and Analysis; 7. Summary; Acknowledgments; References; Chapter Four: Measuring Multivalent Binding Interactions by Isothermal Titration Calorimetry; 1. Introduction; 2. Determining the Thermodynamics of Lectin Binding to Multivalent Synthetic Analogs; 2.1. Methods; 2.1.1. Isothermal Titration Calorimetry 
650 0 |a Calorimetry. 
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650 7 |a Calorimetry.  |2 fast  |0 (OCoLC)fst00844526 
650 1 2 |a Calorimetry. 
655 4 |a Electronic books. 
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700 1 |a Feig, Andrew L.,  |e editor. 
776 0 8 |i Print version:  |a Feig, Andrew  |t Calorimetry  |d : Elsevier Science,c2016  |z 9780128029060 
830 0 |a Methods in enzymology ;  |v v. 567. 
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