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05341nam a2200613 4500 |
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ocn942588196 |
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OCoLC |
| 005 |
20180501122029.0 |
| 006 |
m o d |
| 007 |
cr cnu|||unuuu |
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160302s2016 maua ob 001 0 eng d |
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|a N$T
|b eng
|e rda
|e pn
|c N$T
|d UIU
|d IDEBK
|d N$T
|d CUS
|d YDXCP
|d NLGGC
|d CDX
|d OCLCF
|d EBLCP
|d UPM
|d OTZ
|d UAB
|d OCLCQ
|d CASUM
|d OCLCO
|d NLE
|d D6H
|d OCLCO
|d YDX
|d OCL
|d GrThAP
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| 019 |
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|a 945612328
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|a 9780128036129
|q electronic book
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|a 0128036125
|q electronic book
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|a 9780128035955
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| 035 |
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|a (OCoLC)942588196
|z (OCoLC)945612328
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| 050 |
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4 |
|a QP552.G16
|b G22 2016
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| 050 |
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4 |
|a QH585
|b .M47 v.132
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| 072 |
|
7 |
|a SCI
|x 056000
|2 bisacsh
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|a 572/.696
|2 23
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|a 571.6
|2 23
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| 049 |
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|a TEFA
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| 245 |
0 |
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|a G protein-coupled receptors :
|b signaling, trafficking and regulation /
|c edited by Arun K. Shukla.
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| 250 |
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|a First edition.
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| 264 |
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1 |
|a Cambridge, MA, USA :
|b Academic Press is an imprint of Elsevier,
|c 2016.
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| 300 |
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|a 1 online resource (480 pages) :
|b color illustrations.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in cell biology ;
|v volume 132
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| 504 |
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|a Includes bibliographical references and index.
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|a Front Cover; Series Editors; Methods in Cell Biology G Protein-Coupled Receptors: Signaling, Trafficking and Regulation; Copyright; Contents; Contributors; Preface; 01 -- Trafficking, Localization andImaging of GPCRs; 1 -- Localization and signaling of GPCRs in lipid rafts; INTRODUCTION; 1. LOCALIZATION OF GPCRS IN LIPID RAFTS; 1.1 Isolation of Lipid Rafts; 1.1.1 Detergent-free method; 1.1.2 Detergent-based method; 1.1.3 Immunoblotting and data interpretation; 1.2 Localization of GPCRs in Lipid Rafts; 1.2.1 Cells in suspension; 1.2.2 Adherent cells; 2. GPCR SIGNALING IN LIPID RAFTS
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| 505 |
8 |
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|a 2.1 Perturbation of Raft Stability2.2 Changing the Cholesterol Content; 2.3 Fluorescence Imaging; REFERENCES; 2 -- Imaging GPCRs trafficking and signaling with total internal reflection fluorescence microscopy in cultured neurons; 1. IMAGE ACQUISITION; 1.1 Important Considerations before Imaging; 1.2 Materials; 1.2.1 Cell culture; 1.2.2 TIRF microscopy equipment and settings; 1.3 Imaging; 1.3.1 Cell culture; 1.3.2 Live cell imaging; 1.4 Notes; 2. IMAGE ANALYSIS; 3. FINAL COMMENTS; Acknowledgments; REFERENCES; 3 -- Trafficking of ciliary G protein-coupled receptors; INTRODUCTION
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|a 1. RHODOPSIN AS A CILIARY GPCR2. CILIARY GPCRS IN OTHER NEURONS; 3. OLFACTORY SENSORY NEURON CILIARY GPCRS; 4. DYNAMIC TRAFFICKING OF CILIARY GPCRS; CONCLUSIONS AND FUTURE DIRECTIONS; REFERENCES; 4 -- Single-molecule resolution of G protein-coupled receptor (GPCR) complexes; INTRODUCTION; 1. IMAGING METHODOLOGIES TO STUDY SINGLE GPCR MOLECULES; 1.1 Current Approaches to Visualize Individual GPCRs as Monomers, Dimers, and Oligomers; 1.2 Super-Resolution Imaging of Single Molecules: Resolving Lots and Lots of Spots...; 2. METHODS FOR PHOTOACTIVATED DYE-LOCALIZATION MICROSCOPY
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8 |
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|a 2.1 Seeing Single Molecules2.1.1 Choice of probe and labeling; 2.1.2 Optimization steps-labeling cells, blocking buffers, and fixatives; 2.1.3 Imaging considerations and conditions; 2.2 Counting Single Molecules; 2.2.1 Resolving single-molecule data; 2.2.2 Custom quantification of GPCR monomers, dimers, and oligomers; CONCLUSIONS AND PERSPECTIVES; Acknowledgments; REFERENCES; 5 -- Quantification of the mRNA expression of G protein-coupled receptors in human adipose tissue; INTRODUCTION; 1. MATERIALS; 1.1 Handling of Adipose Tissue Biopsies; 1.2 Adipose Tissue Homogenization
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| 505 |
8 |
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|a 1.3 Adipose Tissue RNA Purification and cDNA Generation1.4 qPCR Quantification of GPCR Expression Relative the Adipose Tissue Housekeeping Gene PPIA; 2. METHODS; 2.1 Handling of Adipose Tissue Biopsies; 2.2 Adipose Tissue Homogenization; 2.3 Adipose Tissue RNA Purification and cDNA Generation; 2.4 qPCR Quantification of GPCR Expression Relative to the Housekeeping Gene PPIA; 2.4.1 Screening of adipose tissue GPCR mRNA expression using pooled cDNA; 2.4.2 Quantification of GPCR expression relative to the expression of the adipose tissue housekeeping gene PPIA
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| 588 |
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|a Description based on online resource; title from digital title page (viewed on March 26, 2018).
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| 650 |
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0 |
|a G proteins
|x Receptors.
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| 650 |
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0 |
|a Cytology
|x Technique.
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| 650 |
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0 |
|a Cell receptors.
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| 650 |
|
7 |
|a SCIENCE / Life Sciences / Anatomy & Physiology
|2 bisacsh
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| 650 |
|
7 |
|a G proteins
|x Receptors.
|2 fast
|0 (OCoLC)fst00936832
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| 650 |
|
7 |
|a Cell receptors.
|2 fast
|0 (OCoLC)fst00850227
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| 650 |
|
7 |
|a Cytology
|x Technique.
|2 fast
|0 (OCoLC)fst00886306
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| 650 |
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2 |
|a GTP-Binding Proteins.
|0 (DNLM)D019204
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| 650 |
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2 |
|a Cytological Techniques.
|0 (DNLM)D003584
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| 650 |
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2 |
|a Receptors, Cell Surface.
|0 (DNLM)D011956
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| 655 |
|
4 |
|a Electronic books.
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| 655 |
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0 |
|a Electronic books.
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| 700 |
1 |
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|a Shukla, Arun K.,
|e editor.
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| 776 |
0 |
8 |
|i Print version:
|a Shukla, Arun K.
|t G Protein-Coupled Receptors: Signaling, Trafficking and Regulation
|d : Elsevier Science,c2016
|z 9780128035955
|
| 830 |
|
0 |
|a Methods in cell biology ;
|v v. 132.
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| 856 |
4 |
0 |
|u https://www.sciencedirect.com/science/bookseries/0091679X/132
|z Full Text via HEAL-Link
|
