Visualizing RNA dynamics in the cell /

• Άλλοι συγγραφείς: Filonov, Grigory S. (Επιμελητής έκδοσης), Jaffrey, Samie R. (Επιμελητής έκδοσης)
• Μορφή: Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: Cambridge, MA : Academic Press is an imprint of Elsevier, 2016.
• Σειρά: Methods in enzymology ; v. 572.
• Θέματα: RNA.; SCIENCE > Life Sciences > Biochemistry.; Electronic books.

 Methods in Enzymology: Visualizing RNA Dynamics in the Cell continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods visualizing RNA dynamics in the cell, and includes sections on such topics as identification of RNA cis-regulatory sequences, IRAS, IMAGEtags, MERFISH, plant RNA labeling using MS2, and visualization of 5S dynamics in live cells using photostable corn probe. 

 Chapter One. RNA Imaging with Multiplexed Error-Robust Fluorescence In Situ Hybridization (MERFISH) / J.R. Moffitt, X. Zhuang -- Chapter Two. Imaging Single mRNA Dynamics in Live Neurons and Brains / H.C. Moon, H.Y. Park -- Chapter Three. Monitoring of RNA Dynamics in Living Cells Using PUM-HD and Fluorescent Protein Reconstitution Technique / H. Yoshimura, T. Ozawa -- Chapter Four. Applications of Hairpin DNA-Functionalized Gold Nanoparticles for Imaging mRNA in Living Cells / S.R. Jackson, A.C. Wong, A.R. Travis, I.E. Catrina, D.P. Bratu, D.W. Wright, A. Jayagopal -- Chapter Five. In Vivo RNA Visualization in Plants Using MS2 Tagging / E.J. Peña, M. Heinlein -- Chapter Six. TRICK: A Single-Molecule Method for Imaging the First Round of Translation in Living Cells and Animals / J.M. Halstead, J.H. Wilbertz, F. Wippich, T. Lionnet, A. Ephrussi, J.A. Chao -- Chapter Seven. Fluctuation Analysis: Dissecting Transcriptional Kinetics with Signal Theory / A. Coulon, D.R. Larson -- Chapter Eight. IMAGEtags: Quantifying mRNA Transcription in Real Time with Multiaptamer Reporters / J. Ray, I. Shin, M. Ilgu, L. Bendickson, V. Gupta, G.A. Kraus, M. Nilsen-Hamilton -- Chapter Nine. A Method for Expressing and Imaging Abundant, Stable, Circular RNAs In Vivo Using tRNA Splicing / C.A. Schmidt, J.J. Noto, G.S. Filonov, A.G. Matera -- Chapter Ten. RNA-ID, a Powerful Tool for Identifying and Characterizing Regulatory Sequences / C.E. Brule, K.M. Dean, E.J. Grayhack -- Chapter Eleven. Fluorescent Protein-Based Quantification of Alternative Splicing of a Target Cassette Exon in Mammalian Cells / N.G. Gurskaya, D.B. Staroverov, K.A. Lukyanov -- Chapter Twelve. IRAS: High-Throughput Identification of Novel Alternative Splicing Regulators / S. Zheng -- Chapter Thirteen. Analysis of Nonsense-Mediated mRNA Decay at the Single-Cell Level Using Two Fluorescent Proteins / N.G. Gurskaya, A.P. Pereverzev, D.B. Staroverov, N.M. Markina, K.A. Lukyanov -- Chapter Fourteen. Developing Fluorogenic Riboswitches for Imaging Metabolite Concentration Dynamics in Bacterial Cells / J.L. Litke, M. You, S.R. Jaffrey.