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05640nam a2200577 4500 |
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ocn957490887 |
| 003 |
OCoLC |
| 005 |
20180501122032.0 |
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m o d |
| 007 |
cr cnu|||unuuu |
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160829s2016 maua ob 001 0 eng d |
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|a N$T
|b eng
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|c N$T
|d BUF
|d OPELS
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|d OCLCQ
|d OCLCO
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|d MERUC
|d OCLCQ
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|a 957598360
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|a 9780128054352
|q (electronic bk.)
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| 020 |
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|a 0128054352
|q (electronic bk.)
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|a 9780128053829
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|a 0128053828
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|a (OCoLC)957490887
|z (OCoLC)957598360
|z (OCoLC)957738099
|z (OCoLC)960214394
|z (OCoLC)970392996
|z (OCoLC)973001938
|z (OCoLC)980831280
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4 |
|a QH225
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| 072 |
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7 |
|a NAT
|x 027000
|2 bisacsh
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7 |
|a SCI
|x 008000
|2 bisacsh
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|a SCI
|x 086000
|2 bisacsh
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0 |
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|a 570.28/2
|2 23
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| 049 |
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|a TEFA
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| 245 |
0 |
4 |
|a The resolution revolution :
|b recent advances in cryoEM /
|c edited by R.A. Crowther.
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| 264 |
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1 |
|a Cambridge, MA :
|b Academic Press is an imprint of Elsevier,
|c 2016.
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| 300 |
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|a 1 online resource :
|b illustrations.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in enzymology ;
|v volume 579
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| 504 |
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|a Includes bibliographical references and indexes.
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| 588 |
0 |
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|a Online resource; title from PDF title page (ScienceDirect, viewed September 1, 2016).
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0 |
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|a Front Cover; The Resolution Revolution: Recent Advances In cryoEM; Copyright; Contents; Contributors; Preface; References; Chapter One: Direct Electron Detectors; 1. Introduction; 2. Past; 3. Present; 3.1. Practical Advice for the User; 4. Future; References; Chapter Two: Specimen Behavior in the Electron Beam; 1. Introduction; 2. High-Energy Electrons Are a Form of Ionizing Radiation as Well as Being a Form of Short-Wavelength Radiation That Can B ... ; 2.1. Electron-Scattering Events Can Be Either Elastic or Inelastic.
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| 505 |
8 |
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|a 2.2. Energy Is Deposited in the Specimen as a Result of Inelastic Scattering2.3. Values of the Linear Energy Transfer (LET) Can Be Used to Estimate the Energy Deposited; 3. Biological Molecules Become Structurally Damaged When Irradiated; 3.1. There Is a Large Literature of Radiation Chemistry and Radiation Biology; 3.2. Fading of Diffraction Patterns Is a Convenient Indicator of Structural Damage; 3.3. Some Residues in Proteins Are Especially Sensitive to Radiation Damage; 3.4. Caging of Fragments and ``Trapping ́́of Radicals Results in Cryo-Protection: This Helps Only to a Limited Extent.
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| 505 |
8 |
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|a 3.5. Radiation Sensitivity of Enzyme Activity: Implications for Dynamic Studies in Liquid Samples4. Vitreous Ice Also Becomes Structurally Damaged by Ionizing Radiation; 4.1. Water Molecules Are Easily Damaged by Ionizing Radiation; 4.2. Weak Thon Rings at High Resolution Show That Vitreous Ice Is Very Sensitive to Radiation Damage; 4.3. Electron-Stimulated Desorption Progressively Thins Ice Specimens; 5. Bubbling of Hydrated Biological Specimens Becomes Apparent at High Electron Exposure; 5.1. Bubbles Consist of Molecular Hydrogen; 5.2. Bubbling Can Be Used to Evaluate the Specimen Thickness.
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| 505 |
8 |
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|a 5.3. Bubbling Can Be Used to Distinguish Regions with Different Chemical Composition (Bubblegrams)6. Cryo-Specimens Exhibit Collective Beam-Induced Movement When Irradiated; 6.1. Radiation-Sensitive Specimens Show Beam-Induced Motion at Quite Low Electron Exposures; 6.2. Thin Cryo-Specimens Undergo Drum-Head-Like Flexing and Doming When Irradiated; 6.3. Images Can Be Corrupted Significantly by There Being Changes in Z-Height; 6.4. The Pattern of Beam-Induced Movement Can Be Quite Unpredictable; 7. More Than One Mechanism May Contribute to Beam-Induced Motion.
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| 505 |
8 |
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|a 7.1. Cryo-EM Specimens, as Made, Are Expected to Be Under Considerable Stress7.2. Irradiation Can Relieve Mechanical Stress; 7.3. Irradiation Can Generate (New) Mechanical Stress; 7.4. Which Comes First, Relaxation or Creation of Stress?; 8. Irradiation Can Produce Electrostatic Charging of the Specimen; 8.1. A Buildup of Net Charge on the Specimen Can Be Easy to Detect; 8.2. Evidence of Net-Charge Buildup Can Be Reduced in Several Ways; 8.3. Other Forms of Specimen Charging Are More Subtle to Detect; 9. Summary and Future Directions; Acknowledgments; References.
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| 650 |
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0 |
|a Cryomicroscopy.
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| 650 |
|
7 |
|a NATURE
|x Reference.
|2 bisacsh
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| 650 |
|
7 |
|a SCIENCE
|x Life Sciences
|x Biology.
|2 bisacsh
|
| 650 |
|
7 |
|a SCIENCE
|x Life Sciences
|x General.
|2 bisacsh
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| 650 |
|
7 |
|a Cryomicroscopy.
|2 fast
|0 (OCoLC)fst00884498
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| 650 |
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7 |
|a Kryoelektronenmikroskopie
|2 gnd
|0 (DE-588)4500745-7
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| 650 |
1 |
2 |
|a Cryoelectron Microscopy.
|0 (DNLM)D020285
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| 655 |
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4 |
|a Electronic books.
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| 655 |
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7 |
|a Electronic books.
|2 local
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| 700 |
1 |
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|a Crowther, R. A.
|q (Roy A.),
|e editor.
|
| 776 |
0 |
8 |
|i Print version:
|z 9780128054352
|
| 830 |
|
0 |
|a Methods in enzymology ;
|v v. 579.
|
| 856 |
4 |
0 |
|u https://www.sciencedirect.com/science/bookseries/00766879/579
|z Full Text via HEAL-Link
|
