Single-molecule enzymology: fluorescence-based and high-throughput methods /

• Άλλοι συγγραφείς: Spies, Maria (Επιμελητής έκδοσης), Chemla, Yann R. (Επιμελητής έκδοσης)
• Μορφή: Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: Cambridge, MA : Elsevier : Academic Press, 2016.
• Σειρά: Methods in enzymology ; v. 581.
• Θέματα: Fluorescence spectroscopy > Congresses.; Fluorescent probes > Congresses.; Luminescence spectroscopy > Congresses.; Luminescent probes > Congresses.; SCIENCE / Chemistry / Analytic; Fluorescence spectroscopy.; Fluorescent probes.; Luminescence spectroscopy.; Luminescent probes.; Spectrometry, Fluorescence.; Microscopy, Fluorescence.; Electronic books.; Conference papers and proceedings.

Πίνακας περιεχομένων:

• Chapter 1. Direct fluorescent imaging of translocation and unwinding by individual DNA helicases
• Chapter 2. Single-molecule imaging with one color fluorescence
• Chapter 3. Measuring membrane protein dimerization equilibrium in lipid bilayers by single-molecule fluorescence microsopy
• Chapter 4. Fluorescent labeling of proteins in whole cell extracts for single-molecule imaging
• Chapter 5. Quantifying the assembly of multicomponent molecular machines by single-molecule total internal reflection fluorescence microscopy
• Chapter 6. How to measure separations and angles between intramolecular fluorescent markers
• Chapter 7. Precisely and accurately inferring single-molecule rate constants
• Chapter 8. Quantification of functional dynamics of membrane proteins reconstituted in nanodiscs membranes by single turnover functional readout
• Chapter 9. Putting Humpty-Dumpty together: clustering the functional dynamics of single biomolecular machines such as the splicosome
• Chapter 10. Single-molecule FRET to measure conformational dynamics of DNA mismatch repair proteins
• Chapter 11. Single-molecule confocal FRET microscopy to dissect conformational changes in the catalytic cycle of DNA topoisomerases
• Chapter 12. Probing the conformational landscape of DNA polymerases using diffusion-based single-molecule FRET
• Chapter 13. Methods for investigating DNA accessibility with single nucleosomes
• Chapter 14. Single-molecule fluorescence studies of fast protein folding
• Chapter 15. Single-molecule multicolor FRET assay for studying structural dynamics of biomolecules
• Chapter 16. A multicolor single-molecule FRET approach to study protein dynamics and interactions simultaneously
• Chapter 17. Interferometric scattering microscopy for the study of molecular motors
• Chapter 18. Enzyme kinetics in femtoliter arrays
• Author index
• Subject index.