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05847nam a2200589 4500 |
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ocn987910300 |
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OCoLC |
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20180501122036.0 |
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m o d |
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cr cnu---unuuu |
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170524s2017 ne af ob 000 0 eng d |
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|d OPELS
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|d YDX
|d EBLCP
|d CASUM
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|d OCLCQ
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|d OCLCO
|d D6H
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|a 987893362
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|a 9780128099759
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|a 9780128099766
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|a 0128099763
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|a (OCoLC)987910300
|z (OCoLC)987893362
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|a QH212.E4
|b C677 2017eb
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|a W1
|b ME9615D v.140 2017
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|a QH 212.E4
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|a 502.8/25
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|a TEFA
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|a Correlative light and electron microscopy III /
|c edited by Thomas Müller-Reichert [and] Paul Verkade.
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|a Amsterdam, Netherlands ;
|a Boston, Mass. :
|b Elsevier / Academic Press,
|c [2017]
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|c ©2017
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| 300 |
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|a 1 online resource (xvii, 352 pages) :
|b illustrations (some color).
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|a text
|b txt
|2 rdacontent
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|a computer
|b c
|2 rdamedia
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|a online resource
|b cr
|2 rdacarrier
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|a Methods in cell biology,
|x 0091-679X ;
|v volume 140
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| 504 |
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|a Includes bibliographical references.
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|a The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy; CLEM has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. This new volume of Methods in Cell Biology covers many areas of CLEM including a brief history and overview on CLEM methods, imaging of intermediate stages of meiotic spindle assembly in C. elegans embryos using CLEM, and capturing endocytic segregation events with HPF-CLEM. Covers many areas of CLEM by the best international scientists in the field. Includes a brief history and overview on CLEM methods.
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|a Print version record.
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|a Front Cover; Methods in Cell Biology; Series Editors; Methods in CellBiology: Correlative Light and Electron Microscopy III; Copyright; Contents; Contributors; Preface: The Key Lies in the Right Combination; 1 -- Millisecond time resolution correlative light and electron microscopy for dynamic cellular processes; INTRODUCTION; 1. METHODS; 1.1 Chlamydomonas reinhardtii Culture; 1.2 Imaging and Embedding Chamber Preparation; 1.3 Light Microscopy; 1.4 Sample Preparation for Electron Microscopy; 1.4.1 Chemical postfixation and embedding; 1.4.2 Thin sectioning
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|a 1.4.3 Staining of the sections and application of fiducial markers1.5 Electron Tomography; 1.6 Registration of Light and Electron Microscopy Images; 1.7 Data Analysis; 1.8 Preparation of Flagellar Cross Sections for Correlative Light and Electron Microscopy Analysis; 2. INSTRUMENTATION AND MATERIALS; 2.1 Chlamydomonas reinhardtii culture; 2.2 Imaging and Embedding Chamber Preparation; 2.3 Light Microscopy; 2.4 Sample Preparation for Electron Microscopy; 2.4.1 Chemical postfixation and embedding; 2.4.2 Thin sectioning; 2.4.3 Staining of the sections and application of fiducial markers
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|a 2.5 Electron Tomography2.6 Registration of Light and Electron Microscopy Images; 2.7 Data Analysis; 2.8 Preparation of Flagellar Cross Sections for Correlative Light and Electron Microscopy Analysis; 3. DISCUSSION AND OUTLOOK; Acknowledgments; REFERENCES; 2 -- 3D subcellular localization with superresolution array tomography on ultrathin sections of various species; INTRODUCTION AND RATIONALE; 1. METHODS-CORE PROTOCOL; 1.1 High-Pressure Freezing; 1.2 Freeze Substitution; 1.3 Embedding; 1.4 Ultramicrotomy; 1.5 Light Microscopy; 1.6 Contrasting and Carbon Coating
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|a 1.7 Scanning Electron Microscopy1.8 Image Processing and Correlation of Structured Illumination Microscopy and Scanning Electron Microscopy Images; 1.9 Alignment and 3D Reconstruction; 1.9.1 IMOD; 1.9.2 Fiji and AMIRA; 2. METHODS-MODEL-SPECIFIC ADAPTATIONS AND CONSIDERATIONS; 2.1 Caenorhabditis elegans; 2.2 Trypanosoma brucei; 2.3 Social Insects (Apis mellifera and Cataglyphis fortis); 3. INSTRUMENTATION AND MATERIALS; 3.1 High-Pressure Freezing; 3.1.1 General; 3.1.2 Caenorhabditis elegans; 3.1.3 Trypanosoma brucei; 3.1.4 Cataglyphis fortis and Apis mellifera; 3.2 Freeze Substitution
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|a 3.3 Embedding3.4 Ultramicrotomy; 3.5 Light Microscopy; 3.5.1 Primary Antibodies; 3.6 Contrasting and Carbon Coating; 3.7 Scanning Electron Microscopy; 3.8 Image Processing and Correlation of Structured Illumination Microscopy and Scanning Electron Microscopy Images; 3.9 Alignment and 3D Reconstruction; CONCLUSIONS; Acknowledgments; REFERENCES; 3 -- Preserving the photoswitching ability of standard fluorescent proteins for correlative in-resin super-resolutio ...; INTRODUCTION; 1. RATIONALE; 2. MATERIALS; 2.1 Instrumentation; 2.2 Materials; 2.3 Chemicals; 3. METHODS; 3.1 Reagent Preparation
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| 650 |
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|a Electron microscopy
|x Technique.
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| 650 |
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7 |
|a SCIENCE
|x Electron Microscopes & Microscopy.
|2 bisacsh
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| 650 |
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7 |
|a Electron microscopy
|x Technique.
|2 fast
|0 (OCoLC)fst00906689
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| 650 |
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7 |
|a SCIENCE / General
|2 bisacsh
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| 650 |
1 |
2 |
|a Microscopy, Electron
|x methods.
|0 https://id.nlm.nih.gov/mesh/D008854Q000379
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| 650 |
2 |
2 |
|a Light.
|0 https://id.nlm.nih.gov/mesh/D008027
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| 655 |
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|a Electronic books.
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| 700 |
1 |
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|a Müller-Reichert, Thomas,
|d 1962-
|e editor.
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| 700 |
1 |
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|a Verkade, Paul,
|e editor.
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| 776 |
0 |
8 |
|i Print version:
|t Correlative light and electron microscopy.
|d Amsterdam, Netherlands ; Boston, Mass. : Elsevier / Academic Press, ©2017
|z 9780128099759
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| 830 |
|
0 |
|a Methods in cell biology ;
|v v. 140.
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| 856 |
4 |
0 |
|u https://www.sciencedirect.com/science/bookseries/0091679X/140
|z Full Text via HEAL-Link
|
