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04461nam a2200589 4500 |
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on1005220362 |
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OCoLC |
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20180501122037.0 |
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m o d |
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cr cnu---unuuu |
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171004s2017 maua ob 001 0 eng d |
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|a N$T
|b eng
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|e pn
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|d OPELS
|d IDEBK
|d EBLCP
|d OCLCO
|d UAB
|d UPM
|d DKU
|d MEU
|d D6H
|d GrThAP
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|c (S
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|a 1029441613
|a 1029505176
|a 1031318407
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|a 9780128133200
|q (electronic bk.)
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|a 0128133201
|q (electronic bk.)
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|a 9780128133194
|q (print)
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|a 0128133198
|q (print)
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|a (OCoLC)1005220362
|z (OCoLC)1029441613
|z (OCoLC)1029505176
|z (OCoLC)1031318407
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|a QP552.G16
|b G67 2017eb
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| 072 |
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7 |
|a SCI
|x 056000
|2 bisacsh
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|a 571.6
|2 23
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| 049 |
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|a TEFA
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| 245 |
0 |
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|a G Protein-Coupled Receptors.
|n Part A /
|c edited by Arun K. Shukla.
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| 250 |
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|a Second edition.
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| 264 |
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1 |
|a Cambridge, MA, USA :
|b Academic Press is an imprint of Elsevier,
|c [2017]
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| 264 |
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4 |
|c Ã2017
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| 300 |
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|a 1 online resource (xiii, 245 pages :
|b color illustrations).
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| 336 |
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|a text
|b txt
|2 rdacontent
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in cell biology ;
|v volume 142
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| 546 |
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|a Text in English.
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|a Includes bibliographical references and index.
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0 |
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|a Print version record.
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0 |
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|a Front Cover; G Protein-Coupled Receptors Part A; Copyright; Contents; Contributors; Preface; Chapter 1: Measurement of GPCR-G protein activity in living cells; 1. Introduction; 2. Materials; 2.1. Generations of Sensors; 2.2. Cell Culture; 2.3. Transfection of Cells; 2.4. Stable Cell Line Generation; 2.5. Live-Cell Imaging; 2.6. Fluorescence Imaging Plate Reader Assay; 3. Methods; 3.1. Generations of Sensors Using Gibson Assembly Protocol; 3.2. Cell Culture; 3.2.1. HeLa Cell-Specific Growth Media Preparation; 3.2.2. Passaging cells
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8 |
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|a 2.1.1. ALPHAscreen (Amplified Luminescent Proximity Homogenous Assay)2.1.2. DELFIA (Dissociation-Enhanced Lanthanide Fluorescence Immunoassay); 2.1.3. HTRF (Homogenous Time Resolved Fluorescence); 2.1.4. Fluorescence polarization assay; 2.2. Luminescence-Based cAMP Measuring Assays; 2.2.1. CRE-luciferase reporter gene assay; 2.2.2. GloSensor assay; 3. Reagents and Consumables; 4. Methods; 4.1. Cell Culture Maintenance and Trypsinization; 4.2. Transient Transfection of HEK293T Cells; 4.3. Preparation of Transfected Cell Plate; 4.4. Test Compound Dilution for GloSensor Assay
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8 |
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|a 4.5. Test Compound Addition to the Cell Plate4.6. Compound Addition to Screen Ligands for GS-Coupled Receptors; 4.7. Compound Addition to Screen Ligands for Gi-Coupled Receptors; 5. Data Analysis; 5.1. Data Analysis for Agonist Activity; 5.2. Data Analysis for Antagonist Activity; 6. Results and Data Interpretation; 7. Final Comments; Acknowledgments; References; Chapter 3: cAMP assays in GPCR drug discovery; 1. Introduction; 2. Materials; 2.1. Cell Culture; 3. Method; 3.1. Troubleshooting; 3.2. Notes; References
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8 |
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|a Chapter 4: Measurement of intracellular Ca2+ mobilization to study GPCR signal transduction1. Introduction; 2. Materials; 2.1. Maintenance of Cell Lines; 2.2. Generation of "Stable Cell Lines"; 2.3. Fura2AM Loading and Spectrophotometric Analysis; 3. Methods; 3.1. Maintenance of Cell Lines; 3.2. Generation of "Stable Cell Lines"; 3.3. Fura2AM Loading; 3.4. Spectrophotometric Analysis; 4. Notes; References; Chapter 5: Visualization and quantification of GPCR trafficking in mammalian cells by confocal microscopy; 1. Introduction; 2. Methods; 2.1. Required Materials
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| 650 |
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0 |
|a Cytology
|x Technique.
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| 650 |
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|a Cell receptors.
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| 650 |
|
7 |
|a SCIENCE / Life Sciences / Anatomy & Physiology
|2 bisacsh
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| 650 |
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7 |
|a Cell receptors.
|2 fast
|0 (OCoLC)fst00850227
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| 650 |
|
7 |
|a Cytology
|x Technique.
|2 fast
|0 (OCoLC)fst00886306
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| 650 |
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2 |
|a GTP-Binding Proteins.
|0 (DNLM)D019204
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| 650 |
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2 |
|a Cytological Techniques.
|0 (DNLM)D003584
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| 650 |
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2 |
|a Receptors, Cell Surface.
|0 (DNLM)D011956
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| 655 |
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4 |
|a Electronic books.
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| 700 |
1 |
|
|a Shukla, Arun K.,
|e editor.
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| 776 |
0 |
8 |
|i Print version:
|t G protein-coupled receptors.
|b Second edition.
|d Cambridge, MA, USA : Academic Press is an imprint of Elsevier, 2017
|z 9780128133194
|
| 830 |
|
0 |
|a Methods in cell biology ;
|v v. 142.
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| 856 |
4 |
0 |
|u https://www.sciencedirect.com/science/bookseries/0091679X/142
|z Full Text via HEAL-Link
|
