Fe-S Cluster Enzymes. Part B /

Λεπτομέρειες βιβλιογραφικής εγγραφής
Άλλοι συγγραφείς: David, Sheila S. (Επιμελητής έκδοσης)
Μορφή: Ηλ. βιβλίο
Γλώσσα:English
Έκδοση: Cambridge, Massachusetts : Academic Press, 2018.
Έκδοση:First edition.
Σειρά:Methods in enzymology ; vol. 599.
Θέματα:
Διαθέσιμο Online:Full Text via HEAL-Link
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245 0 0 |a Fe-S Cluster Enzymes.  |n Part B /  |c edited by Sheila S. David. 
250 |a First edition. 
260 |a Cambridge, Massachusetts :  |b Academic Press,  |c 2018. 
300 |a 1 online resource (510 pages) 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
338 |a online resource  |b cr  |2 rdacarrier 
347 |a data file  |2 rda 
490 1 |a Methods in enzymology ;  |v vol. 599 
504 |a Includes bibliographical references and indexes. 
588 0 |a Print version record. 
505 0 |a Front Cover; Fe-S Cluster Enzymes Part B; Copyright; Contents; Contributors; Preface; Chapter One: Iron-Sulfur Clusters in DNA Polymerases and Primases of Eukaryotes; 1. Introduction; 2. Iron-Sulfur Clusters in DNA Polymerases and Primase; 2.1. DNA Polymerases at the Replication Fork; 2.2. Iron-Sulfur Cluster in the Large Subunit of DNA Primase; 2.3. Iron-Sulfur Clusters in B-Family DNA Polymerases; 3. Genetic Evidence for the Important Roles of Iron-Sulfur Clusters in DNA Replication In Vivo; 4. Purification of Eukaryotic B-Family DNA Polymerases; 4.1. Procedure; 4.2. Notes. 
505 8 |a 5. Analysis of Iron Content in Protein Samples5.1. Procedure; 5.2. Notes; Acknowledgment; References; Chapter Two: Fe-S Clusters and MutY Base Excision Repair Glycosylases: Purification, Kinetics, and DNA Affinity Measurements; 1. Introduction; 2. Over Expression and Purification of MutY Homologs; 2.1. Considerations for Isolating MutY Homologs; 2.2. Bacteria as an Overexpression Host; 2.3. Purification of E. coli MutY; 2.4. Purification of a Thermophilic Homolog, G. stearothermophilus MutY; 2.5. MBP-MutY for Higher Yields and Solubility; 2.6. Bacterial Expression of M. musculus Mutyh. 
505 8 |a 2.7. Eukaryotic Expression System for Production of Homo sapiens MUTYH3. Gel-Based Adenine Glycosylase Assays and Measurements of Kinetic Parameters; 3.1. General Setup and Execution of the Glycosylase Assay; 3.1.1. Radiolabeling the DNA Substrate; 3.1.2. Preparation of a Denaturing Polyacrylamide Gel; 3.1.3. General Assay Setup; 3.1.4. Visualization and Quantitation of Results; 3.1.5. Salt Concentration in Assay Buffer; 3.2. Correcting the Enzyme Concentration for the Percent Active Fraction; 3.3. Determining the Rate of Product Release; 3.4. Assessing the Rate of Glycosidic Bond Cleavage. 
505 8 |a 4. Gel-Based Assays for Determining MutY-DNA Affinity4.1. General Features and Considerations of Gel-Based Binding Assays With MutY; 4.1.1. Radiolabeling the DNA Substrate; 4.1.2. Preparation of a Nondenaturing Polyacrylamide Gel; 4.1.3. Running an EMSA; 4.1.4. Visualization and Quantitation of Results; 4.2. Measurements of MutY-DNA Dissociation Constants; 4.3. Measurement of DNA Dissociation Rate (koff); 5. Application of Methods to Reveal Roles of the Fe-S Cluster Cofactor in MutY Homologs; Acknowledgments; References. 
505 8 |a Chapter Three: Cellular Assays for Studying the Fe-S Cluster Containing Base Excision Repair Glycosylase MUTYH and Homologs1. Introduction; 2. Mutation Suppression Activity Measured in Rifampicin Resistance Assays; 2.1. Overview of the Rifampicin Resistance Assay; 2.2. Transformation and Growth of Cells; 2.3. Determining the Mutation Frequency; 2.3.1. Troubleshooting Tips; 2.4. Case Study: Use of Rifampicin Resistance Assay to Characterize Zn-Linchpin Motif in MUTYH; 3. Analysis of MutY-Mediated Repair of Defined Plasmid Substrates in E. coli. 
500 |a 3.1. Designing a Plasmid-Based Bacterial Cell Assay. 
650 0 |a Iron-sulfur proteins. 
650 2 |a Iron-Sulfur Proteins.  |0 (DNLM)D007506 
650 7 |a SCIENCE  |x Life Sciences  |x Biochemistry.  |2 bisacsh 
650 7 |a Iron-sulfur proteins.  |2 fast  |0 (OCoLC)fst01721284 
655 4 |a Electronic books. 
700 1 |a David, Sheila S.,  |e editor. 
776 0 8 |i Print version:  |a David, Sheila S.  |t Fe-S Cluster Enzymes Part B.  |d San Diego : Elsevier Science, ©2018  |z 9780128147177 
830 0 |a Methods in enzymology ;  |v vol. 599. 
856 4 0 |3 ScienceDirect  |u https://www.sciencedirect.com/science/bookseries/00766879/599  |z Full Text via HEAL-Link