Mechanisms of DNA recombination and genome rearrangements : methods to study homologous recombination /

• Άλλοι συγγραφείς: Spies, Maria (Επιμελητής έκδοσης), Malkova, Anna (Επιμελητής έκδοσης)
• Μορφή: Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: Cambridge, MA : Academic Press is an imprint of Elsevier, 2018.
• Σειρά: Methods in enzymology ; v. 600.
• Θέματα: Genetic recombination.; Recombinant DNA.; Recombination, Genetic.; DNA, Recombinant.; SCIENCE / Life Sciences / Biochemistry; Electronic books.

 Mechanisms of DNA Recombination and Genome Rearrangements: Intersection between Homologous Recombination, DNA Replication and DNA Repair, Volume 601, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Homologous genetic recombination remains the most enigmatic process in DNA metabolism. The molecular machines of recombination preserve the integrity of the genetic material in all organisms and generate genetic diversity in evolution. The same molecular machines that support genetic integrity by orchestrating accurate repair of the most deleterious DNA lesions, however, also promote survival of cancerous cells and emergence of radiation and chemotherapy resistance. This two-volume set offers a comprehensive set of cutting edge methods to study various aspects of homologous recombination and cellular processes that utilize the enzymatic machinery of recombination. The chapters are written by the leading researches and cover a broad range of topics from the basic molecular mechanisms of recombinational proteins and enzymes to emerging cellular techniques and drug discovery efforts. 

 1. DSB processing in yeast / Loraine Symington -- 2. Purification of DNA end resection enzymes / Petr Ceijka -- 3. Biochemistry of DNA end resection / Petr Ceijka -- 4. Visualization of telomere movement and interchromosomal homology searches in ALT / Roger Greenberg -- 5. Pre-steady state kinetic methods for investigating the DNA exonuclease activity of Mre11 / Scott Nelson -- 6. Production of large quantities of human RAD51 / Maria Spies -- 7. Determining the RAD51 nucleoprotein filamentous structure and mechanism by CryoEM / Hong-Wei Wang -- 8. Single-molecule TIRF analysis of human RAD51 nucleation / Maria Spies -- 9. TIRF-based single-molecule detection of the RecA presynaptic filament dynamics / Sung Hyun Kim -- 10. Archaeal (Sulpholobus) RadA and its paralogs / Cynthia Haseltine -- 11. 4-strand DNA strand exchange / Alexander Mazin -- 12. Purification and biochemistry of DMC1 recombinase / Doug Bishop -- 13. Single-molecule TIRF analysis of yeast Rad51 nucleoprotein disassembly by helicases / Sua Myong -- 14. The recombination mediator BRCA2: architectural plasticity of recombination intermediates revealed by single molecule imaging (SFM/TIRF) / Claire Wyman -- 15. Visualisation and quantitation of BRCA2/RAD51 dynamics in cells / Roland Kanaar -- 16. RPA -- DNA curtains / Eric Greene -- 17. Single-molecule studies of ssDNA binding proteins / Taekjip Ha -- 18. BRCA2 as recombination mediator / Aura Carreira -- 19. Ustilago maydis Brh2 as a model for human BRCA2 / Bill Holloman -- 20. Purification and analysis of holiday junction resolvases / Stephen West -- 21. Structural analysis of HJ resolving enzyme GEN1 / David Lilley -- 22. The preparation of four-stranded recombination intermediates for use in the HJ resolution reactions / Stephen West.