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m o d |
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180529s2018 mau o 000 0 eng d |
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|d OPELS
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|a 018883646
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|a 1038059216
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|a 9780128141458
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|a 9780128141441
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|a 0128141441
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|z (OCoLC)1038059216
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|a QH605
|b .M5 2018
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|2 23
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|a Mitosis and meiosis.
|n Part A /
|c edited by Helder Maiato, Melina Schuh.
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| 264 |
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1 |
|a Camridge, MA :
|b Academic Press,
|c 2018.
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| 300 |
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|a 1 online resource
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| 336 |
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|a text
|b txt
|2 rdacontent
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|a computer
|b c
|2 rdamedia
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in cell biology ;
|v volume 144
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|a Online resource; title from PDF title page (ScienceDirect, viewed May 30, 2018).
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|a Front Cover; Mitosis and Meiosis Part A; Copyright; Contents; Contributors; Preface; Chapter 1: Assays for the spindle assembly checkpoint in cell culture; 1. Introduction; 2. Choice of Fluorescent Protein Tag; 3. Choice of Microscope; 4. Assays; 5. Materials; Acknowledgments; References; Chapter 2: Quantitative methods to measure aneuploidy and chromosomal instability; 1. Introduction; 2. Immunofluorescence Assay for Lagging Chromosomes in Anaphase; 2.1. Instrumentation; 2.2. Cell Culture Conditions; 2.3. Antibodies; 2.4. Immunofluorescence; 2.5. Analysis; 2.6. Alternative Method
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|a 3. Chromosome Missegregation Assay3.1. Instrumentation; 3.2. Cell Culture Conditions; 3.3. FISH Probes; 3.4. Preparation of Mitotic Cells; 3.5. FISH Procedure; 3.6. Analysis; 3.7. Alternative Method; 4. Aneuploid Cell Survival Assay; 4.1. Instrumentation; 4.2. Cell Culture Conditions; 4.3. FISH Probes; 4.4. Preparation of Interphase Cells; 4.5. FISH Procedure; 4.6. Analysis; 4.7. Alternative Method; 5. Concluding Remarks; Acknowledgments; References; Chapter 3: Dissecting the role of the tubulin code in mitosis; 1. Introduction; 1.1. What Is the Tubulin Code?; 1.2. Tubulin Isotypes
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|a 1.3. Tubulin PTMs1.4. How Is the Tubulin Code Read?; 2. Modulation of the Detyrosination/Tyrosination Cycle in Mammalian Cells; 2.1. Cell Culture; 2.2. Transient Overexpression of TTL; 2.3. Depletion of TTL Using Small Interference RNAs (siRNAs); 2.4. Knockout of TTL Using CRISPR/Cas9; 2.4.1. Purchase oligos; 2.4.2. Oligo annealing and cloning into viral transfer vectors; 2.4.3. Transformation and selection; 2.4.4. Lentivirus production; 2.4.5. Transduction of lentivirus to target cells; 2.4.6. Selection of knockout cells; 2.5. Transient Overexpression of VASH1 and VASH2
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|a 2.6. Generation of Cell Lines Stably Expressing FLAG-VASH1 and FLAG-VASH22.6.1. Retrovirus production; 2.6.2. Transduction of retrovirus to target cells and selection of overexpressing cells; 2.7. Knockout of VASH1 and VASH2 Using CRISPR/Cas9; 2.7.1. Purchase oligos; 2.7.2. Oligo annealing and cloning into viral transfer vectors; 2.7.3. Lentivirus production; 2.7.4. Transduction of lentivirus to target cells and selection of knockout cells; 2.8. Depletion of Endogenous α-Tubulin Isotypes Using siRNA; 2.9. Transient Overexpression of Tyrosinated, Detyrosinated and Delta2 Forms of TUBA1B
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|a 2.9.1. Site-directed mutagenesis of mammalian expression vectors2.9.2. Altering the cDNA sequences to confer resistance to siRNA depletion; 2.9.3. Transient expression of tyrosinated, detyrosinated, and Delta2 forms of TUBA1B; 2.10. Generation of a Cell Line Stably Expressing H2B-mRFP and Tyrosinated, Detyrosinated, or Delta2 Forms of TUBA1B; 2.10.1. Cloning of TUBA1B cDNA into lentiviral transfer vectors; 2.10.2. Deletion of EGFP-tag from lentiviral vectors expressing TUBA1B by PCR; 2.10.3. Production of lentivirus and transduction to target cells
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0 |
|a Mitosis.
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| 650 |
|
0 |
|a Meiosis.
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| 650 |
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2 |
|a Mitosis.
|0 (DNLM)D008938
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| 650 |
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2 |
|a Meiosis.
|0 (DNLM)D008540
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| 650 |
|
7 |
|a SCIENCE
|x Life Sciences
|x Anatomy & Physiology.
|2 bisacsh
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| 650 |
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7 |
|a Meiosis.
|2 fast
|0 (OCoLC)fst01015610
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| 650 |
|
7 |
|a Mitosis.
|2 fast
|0 (OCoLC)fst01024031
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| 655 |
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4 |
|a Electronic books.
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| 700 |
1 |
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|a Maiato, Helder,
|e editor.
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| 700 |
1 |
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|a Schuh, Melina,
|e editor.
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| 776 |
0 |
8 |
|i Print version:
|t Mitosis and meiosis. Part A.
|d Camridge, MA : Academic Press, 2018
|z 0128141441
|z 9780128141441
|w (OCoLC)1020635024
|
| 830 |
|
0 |
|a Methods in cell biology ;
|v v. 144.
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| 856 |
4 |
0 |
|3 ScienceDirect
|u https://www.sciencedirect.com/science/bookseries/0091679X/144
|z Full Text via HEAL-Link
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| 856 |
4 |
0 |
|3 ScienceDirect
|u https://www.sciencedirect.com/science/book/9780128141441
|z Full Text via HEAL-Link
|
