Enzymes in synthetic biology /

• Άλλοι συγγραφείς: Scrutton, Nigel S. (Επιμελητής έκδοσης)
• Μορφή: Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: Cambridge, MA : Academic Press is an imprint of Elsevier, 2018.
• Έκδοση: First edition.
• Σειρά: Methods in enzymology ; v. 608.
• Θέματα: Enzymes.; Synthetic biology.; SCIENCE > Chemistry > Industrial & Technical.; TECHNOLOGY & ENGINEERING > Chemical & Biochemical.; Electronic books.

Volume 608 of the series Methods in Enzymology covers key aspects of enzyme discovery, engineering tools and platforms, and examples of applications in the enzymology of synthetic biology. Detailed methods for laboratory use of enzymes in synthetic biology applicationsInformative case history exampl...

Πίνακας περιεχομένων:

• Intro; Title page; Table of Contents; Copyright; Contributors; Preface; Section I: Enzyme Discovery; Chapter One: Enzyme Discovery: Enzyme Selection and Pathway Design; Abstract; 1 Introduction; 2 Enzyme Selection; 3 Pathway Design; 4 Summary and Conclusion; Acknowledgments; Section II: Enzyme Engineering Tools and Platforms; Chapter Two: Cell-Free Synthetic Biology for Pathway Prototyping; Abstract; 1 Introduction; 2 The Cell-Free Metabolic Engineering Framework; 3 S12 Lysate Preparation for Cell-Free Metabolic Engineering; 4 Mix-and-Match Cell-Free Metabolic Engineering
• 5 Cell-Free Protein Synthesis-Driven Metabolic Engineering6 Summary and Conclusions; Acknowledgments; Chapter Three: Fast and Flexible Synthesis of Combinatorial Libraries for Directed Evolution; Abstract; 1 Introduction; 2 Methods; 3 Library Ligation, Transformation, and Quality Control; 4 Summary and Conclusion; Acknowledgments; Section III: Enzyme Families: Parts and Platforms for Chemical Diversity; Chapter Four: Sesquiterpene Synthase-Catalyzed Conversion of a Farnesyl Diphosphate Analogue to a Nonnatural Terpenoid Ether; Abstract; 1 Introduction
• 2 Sesquiterpenes Production and Purification3 Enzymatic Synthesis of Cyclic Ether; 4 Conclusion; Chapter Five: In Vivo Platforms for Terpenoid Overproduction and the Generation of Chemical Diversity; Abstract; 1 Introduction; 2 In Vitro Reconstitution of the MVA Pathway and Targeted Engineering of Value-Added Terpenoids; 3 Genome Mining of Terpene Cyclases and the Generation of Chemical Diversity; 4 Conclusion; Acknowledgments; Chapter Six: Imine Reductases, Reductive Aminases, and Amine Oxidases for the Synthesis of Chiral Amines: Discovery, Characterization, and Synthetic Applications
• Abstract1 Introduction; 2 Protein Engineering; 3 Biotransformations, Intensification, and Scale-Up; 4 In Vitro and in vivo Cascades; 5 Conclusions; Chapter Seven: Experimental Protocols for Generating Focused Mutant Libraries and Screening for Thermostable Proteins; Abstract; 1 Introduction; 2 Single Mutants Generation; 3 Combining Mutations; 4 Summary and Conclusions; Acknowledgments; Chapter Eight: Characterization of Cytochrome P450 Enzymes and Their Applications in Synthetic Biology; Abstract; 1 Introduction; 2 Expression and Purification of Microbial Cytochrome P450 Enzymes
• 3 Expression and Purification of Eukaryotic P450s in E. coli4 Expression and Characterization of P450 Redox Partner Systems; 5 Determination of P450 Purity, Concentration, and Other Properties Using UV-Visible Spectroscopy; 6 Using Cytochromes P450 for Oxidative Transformations; 7 Crystallization and Structural Analysis of P450S; 8 Applications of P450 Enzymes in Synthetic Biology; 9 Summary and Conclusion; Section IV: Genome Engineering for Enzyme Pathways