Enzymes of energy technology /

• Άλλοι συγγραφείς: Armstrong, Fraser (Επιμελητής έκδοσης)
• Μορφή: Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: United Kingdom : Academic Press, 2018.
• Έκδοση: First edition.
• Σειρά: Methods in enzymology ; v. 613.
• Θέματα: Enzymes > Industrial applications.; SCIENCE > Chemistry > Industrial & Technical.; TECHNOLOGY & ENGINEERING > Chemical & Biochemical.; Electronic books.

Enzymes of Energy Technology, Volume 613 in the Methods in Enzymology series, highlights new advances in the field, with this updated volume presenting interesting chapters written by an international board of authors. Chapters include Purification of fully active and crystallizable photosystem II f...

Πίνακας περιεχομένων:

• Front Cover; Enzymes of Energy Technology; Copyright; Contents; Contributors; Preface; Chapter One: Purification of fully active and crystallizable photosystem II from thermophilic cyanobacteria; 1. Introduction; 2. Growth and harvest of cells; 3. Isolation of thylakoid membranes; 4. Isolation of crude PSII particles; 5. Purification of PSII dimers; 6. Characterization of the purified PSII core dimers; 7. Crystallization of purified PSII dimers; Acknowledgments; References; Chapter Two: Production and manipulation of blue copper oxidases for technological applications; 1. Introduction
• 2. Heterologous production of fungal laccases2.1. Cloning; 2.1.1. Site-directed mutagenesis; 2.1.2. Yeast recombination; 2.2. Transformations; 2.2.1. S. cerevisiae; 2.2.2. A. niger; 2.3. Screening for laccase secreting transformants in S. cerevisiae and A. niger; 2.3.1. Screening of clones from deep-well or microtiter plate cultures; 2.3.2. Screening of laccase producing transformants on petri dish plates; 2.3.3. Screening of activity on 96-well plate; 2.3.4. ELISA screening; 2.4. Fermentation in A. niger; 2.4.1. Preparation of spore inoculum and storage
• 2.4.2. Production in bioreactor and growth parameters3. General purification procedure and characterization; 3.1. Purification procedure; 3.1.1. Assay procedures; 3.1.2. Laccase purification procedure; 4. Applications; 4.1. General procedure for an oriented covalent grafting of the laccase surface; 4.1.1. Preparation of laccase variants for single-site anchoring; 4.1.2. Reductive amination of lysine; 4.1.3. Evaluation of the grafting; 4.2. Hybridization with materials; 5. Conclusions; Acknowledgment; References; Further reading
• Chapter Three: Production and spectroscopic characterization of lytic polysaccharide monooxygenases1. Introduction; 2. Expression and purification of LPMOs; 2.1. Recombinant expression and purification from the E. coli periplasm; 2.2. Recombinant expression and purification from the E. coli cytoplasm; 2.3. Recombinant expression and purification from yeast; 2.4. Recombinant expression and purification from fungi; 3. EPR spectroscopy; 3.1. EPR sample preparation for LPMOs; 3.2. Multifrequency collection; 3.3. Simulation procedures; Acknowledgments; References; Further reading
• Chapter Four: A novel overproduction system for the structural determination of a proton-pumping hydrogen-producing [NiFe ... 1. Introduction; 1.1. Hydrogen catalysis in nature; 1.2. Escherichia coli hydrogenase-2 genetic and structural arrangement; 1.3. First approaches to overexpression of a [NiFe]-hydrogenase; 2. Overproduction of fully formed holo-Hyd-2; 2.1. Molecular biology strategies for the overproduction of Hyd-2; 2.1.1. Design of overexpression plasmid pO; 2.1.1.1. Equipment; 2.1.1.2. Reagents and buffers; 2.1.1.3. Procedures; 2.1.2. Design of overexpression strain HJ001-hyp