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05642nam a2200673 4500 |
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on1088407326 |
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OCoLC |
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20200326101159.0 |
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m o d |
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cr cnu|||unuuu |
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190225s2019 mau ob 000 0 eng d |
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|a N$T
|b eng
|e rda
|e pn
|c N$T
|d N$T
|d OPELS
|d EBLCP
|d YDX
|d UKMGB
|d UKAHL
|d OCLCF
|d OCLCQ
|d CASUM
|d OCLCO
|d OCLCA
|d GrThAP
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|a GBB946377
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|a 019270541
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|a 1088698819
|a 1089117154
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|a 9780128170755
|q (electronic bk.)
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|a 0128170751
|q (electronic bk.)
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|a 9780128170748
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|a 0128170743
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|a (OCoLC)1088407326
|z (OCoLC)1088698819
|z (OCoLC)1089117154
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|a TP248.65.E59
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|a SCI
|x 013060
|2 bisacsh
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|a TEC
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|a 660.6/34
|2 23
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|a Metabolons and supramolecular enzyme assemblies /
|c edited by Claudia Schmidt-Dannert, Maureen B. Quin.
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|a First edition.
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| 264 |
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1 |
|a Cambridge, MA :
|b Academic Press, an imprint of Elsevier,
|c 2019.
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| 300 |
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|a 1 online resource
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in enzymology ;
|v volume 617
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| 504 |
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|a Includes bibliographical references.
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| 588 |
0 |
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|a Online resource; title from PDF title page (EBSCO, viewed February 26, 2019).
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0 |
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|a Front Cover; Metabolons and Supramolecular Enzyme Assemblies; Copyright; Contents; Contributors; Preface; Chapter One: Molecular snapshots of dynamic membrane-bound metabolons; 1. Introduction; 2. SMA copolymer preparation; 2.1. SMA copolymer hydrolysis; 2.1.1. Equipment; 2.1.2. Buffers and reagents; 2.1.3. Procedures; 2.1.4. Notes; 3. Preparation of S. bicolor microsomes; 3.1. Growth of etiolated S. bicolor seedlings; 3.1.1. Equipment; 3.1.2. Buffers and reagents; 3.1.3. Procedures; 3.1.4. Notes; 3.2. Preparation of microsomes; 3.2.1. Equipment; 3.2.2. Buffers and reagents; 3.2.3. Procedures
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| 505 |
8 |
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|a 3.2.4. Notes4. Isolation of membrane-bound metabolons from native membranes; 4.1. SMALP preparation from sorghum microsomes; 4.1.1. Equipment; 4.1.2. Buffers and reagents; 4.1.3. Procedures; 4.1.4. Notes; 4.2. Purification of SMALPs containing a target protein; 4.2.1. Equipment; 4.2.2. Buffers and reagents; 4.2.3. Procedures; 4.2.4. Notes; 5. Characterization of SMALPs containing P450 metabolons; 5.1. SMALPs observed with electron microscope; 5.1.1. Equipment; 5.1.2. Buffers and reagents; 5.1.3. Procedures; 5.1.4. Notes; 6. Summary and conclusion; References
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8 |
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|a Chapter Two: Tricarboxylic acid metabolon1. Introduction; 1.1. Importance of the TCA metabolon; 1.2. Early evidence of the TCA metabolon and challenges associated with its study; 1.3. Cross-linking mass spectrometric analysis of the TCA metabolon; 2. Preparation of cross-linked native TCA metabolon; 2.1. Isolation of intact mitochondria; 2.2. In situ cross-linking; 2.3. Isolation and characterization of metabolons; 3. Preparation of recombinant TCA metabolon; 3.1. Gene construction for recombinant TCA enzymes; 3.2. Overexpression and purification of recombinant enzymes
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|a 3.3. In-solution cross-linking and isolation of recombinant metabolons4. Mass spectrometric analysis of the Krebs cycle metabolon; 4.1. In-gel digestion of metabolons; 4.2. Mass spectrometric analysis; 4.3. Mascot database searches; 4.4. Structural reconstitution of the Krebs cycle metabolon; 5. Cross-linking techniques have allowed for the isolation of the intact metabolon; 6. Conclusions; References; Chapter Three: Metabolon formation by chemotaxis; 1. Introduction; 1.1. Metabolon formation; 1.2. Enzyme diffusion; 1.3. Enzyme chemotaxis; 2. Chemotactic assembly of enzyme cascade
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8 |
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|a 2.1. Chemotactic assembly in the microfluidic channel2.1.1. Fluorescently labeling enzymes and activity assay; 2.1.2. Microfluidic device fabrication; 2.1.3. Confocal microscope imaging; 2.1.4. Chemotactic migration of hexokinase and aldolase in the microfluidic device; 2.2. Chemotactic colocalization of hexokinase and aldolase; 2.2.1. Colocalization setup; 2.2.2. Analysis of HK and Ald aggregates trajectories; 3. Summary; Acknowledgments; References; Chapter Four: Cloning, expression, and purification of intact polyketide synthase modules; 1. Introduction; 2. Cloning of intact PKS modules
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| 650 |
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0 |
|a Enzymes
|x Biotechnology.
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| 650 |
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0 |
|a Enzymology.
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| 650 |
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0 |
|a Supramolecular chemistry.
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| 650 |
1 |
2 |
|a Enzymes
|x metabolism.
|0 (DNLM)D004798Q000378
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| 650 |
1 |
2 |
|a Metabolomics
|x methods.
|0 (DNLM)D055432Q000379
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| 650 |
2 |
2 |
|a Metabolic Networks and Pathways.
|0 (DNLM)D053858
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| 650 |
2 |
2 |
|a Metabolome
|x physiology.
|0 (DNLM)D055442Q000502
|
| 650 |
2 |
2 |
|a Molecular Biology.
|0 (DNLM)D008967
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| 650 |
|
7 |
|a SCIENCE
|x Chemistry
|x Industrial & Technical.
|2 bisacsh
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| 650 |
|
7 |
|a TECHNOLOGY & ENGINEERING
|x Chemical & Biochemical.
|2 bisacsh
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| 650 |
|
7 |
|a Enzymes
|x Biotechnology.
|2 fast
|0 (OCoLC)fst00913608
|
| 650 |
|
7 |
|a Enzymology.
|2 fast
|0 (OCoLC)fst00913635
|
| 650 |
|
7 |
|a Supramolecular chemistry.
|2 fast
|0 (OCoLC)fst01139159
|
| 655 |
|
4 |
|a Electronic books.
|
| 700 |
1 |
|
|a Schmidt-Dannert, Claudia,
|e editor.
|
| 700 |
1 |
|
|a Quin, Maureen B.,
|e editor.
|
| 776 |
0 |
8 |
|i Print version:
|t Metabolons and supramolecular enzyme assemblies.
|b First edition.
|d Cambridge, MA : Academic Press, an imprint of Elsevier, 2019
|z 0128170743
|z 9780128170748
|w (OCoLC)1047775290
|
| 830 |
|
0 |
|a Methods in enzymology ;
|v v. 617.
|
| 856 |
4 |
0 |
|3 ScienceDirect
|u https://www.sciencedirect.com/science/bookseries/00766879/617
|z Full Text via HEAL-Link
|
