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04695nam a2200541 4500 |
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on1100588396 |
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OCoLC |
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20200326101201.0 |
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m o d |
| 007 |
cr cnu---unuuu |
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190509s2019 enk o 000 0 eng d |
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|a N$T
|b eng
|e rda
|e pn
|c N$T
|d N$T
|d EBLCP
|d OPELS
|d UKMGB
|d OCLCF
|d UKAHL
|d OCLCQ
|d CASUM
|d OCLCO
|d OCLCA
|d GrThAP
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|a GBB992187
|2 bnb
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|a 019400244
|2 Uk
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|a 9780128168301
|q (electronic bk.)
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|a 0128168307
|q (electronic bk.)
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|a 9780128168295
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|a (OCoLC)1100588396
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|a QP671.F5
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|a SCI
|x 008000
|2 bisacsh
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|a PSD
|2 bicssc
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|a 572/.7
|2 23
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|a 574.19258
|2 23
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|a Palfey, Bruce,
|e author.
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| 245 |
1 |
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|a New approaches for flavin catalysis /
|c Bruce A. Palfey.
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| 264 |
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1 |
|a London :
|b Academic Press,
|c 2019.
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| 300 |
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|a 1 online resource
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in enzymology ;
|v volume 620
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| 588 |
0 |
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|a Online resource; title from PDF title page (EBSCO, viewed May 10, 2019)
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0 |
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|a Front Cover; New Approaches for Flavin Catalysis; Copyright; Contents; Contributors; Preface; Chapter One: Methods for determining the reduction potentials of flavin enzymes; 1. Introduction; 2. Spectroelectrochemistry method; 3. Xanthine oxidase method; 3.1. Materials; 3.2. Protocol; 3.3. Considerations; 3.4. Data analysis; 4. Effects of ligands; 5. Conclusion; References; Chapter Two: Anaerobic methods for the transient-state study of flavoproteins: The use of specialized glassware to define ... ; 1. Introduction; 1.1. O2 as a substrate or a reactive contaminant
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| 505 |
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|a 1.2. Categories of flavoprotein reactions2. Controlling the concentration of molecular oxygen; 2.1. The limits of anaerobiosis and approach with glove-boxes; 2.2. The advantages of specialized glassware; 2.3. Instrument design considerations; 2.4. The Schlenk line; 2.5. Anaerobic vessels: Tonometers and cuvettes; 2.6. Exchanging argon for dissolved dioxygen: Making a sample anaerobic; 2.7. Anaerobic preparation of the rapid-mixing instrument; 2.8. Method for mounting tonometers and syringes on the kinetic instrument; 2.9. Gas blending and sparging to control the dioxygen concentration
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|a 3. Preparing reduced flavoproteins3.1. Titration with sodium hydrosulfite; 3.2. Double-mixing and reduction using the native reductant; 3.3. Enzymatic reduction using mediators; 3.4. Light mediated reduction; 4. Summary and conclusion; References; Chapter Three: Performing anaerobic stopped-flow spectrophotometry inside of an anaerobic chamber; 1. Introduction; 2. Anaerobic chambers; 2.1. Anaerobic chamber components; 2.2. Maintaining an anaerobic chamber; 2.2.1. Maintaining chamber anaerobiosis; 2.2.2. Maintaining humidity and removing hydrogen sulfide
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|a 2.3. Flexible chambers compared to rigid chambers3. Introduction to stopped-flow spectrophotometry; 3.1. Components of a stopped-flow spectrophotometer; 3.2. Anaerobic stopped-flow spectrophotometry; 4. Setting up, preparing, and performing stopped-flow spectrophotometry assays inside of an anaerobic chamber; 4.1. Setting up a stopped-flow spectrophotometer inside of an anaerobic chamber; 4.1.1. Materials; 4.1.2. Procedure; 4.1.3. Notes; 4.2. Setting up a Schlenk line on a cart; 4.2.1. Equipment; 4.2.2. Procedure; 4.2.3. Notes; 4.3. Preparing anaerobic buffers; 4.3.1. Equipment
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|a 4.3.2. Buffers and reagents4.3.3. Procedure; 4.3.4. Notes; 4.4. Transferring anaerobic solutions; 4.4.1. Equipment; 4.4.2. Procedure; 4.4.3. Notes; 4.5. Addition of enzymes to maintain anaerobic solutions; 4.5.1. Equipment; 4.5.2. Buffers or reagents; 4.5.3. Procedure; 4.5.4. Notes; 4.6. Determining oxygen concentration in deoxygenated buffers to validate anaerobiosis; 4.6.1. Equipment; 4.6.2. Buffers and reagents; 4.6.3. Procedure; 4.6.4. Notes; 4.7. Preparing anaerobic enzyme solutions; 4.7.1. Equipment; 4.7.2. Buffers and reagents; 4.7.3. Procedure; 4.7.4. Notes
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| 650 |
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|a Flavins.
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| 650 |
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|a Flavoproteins.
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| 650 |
1 |
2 |
|a Flavins
|x chemistry.
|0 (DNLM)D005415Q000737
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| 650 |
2 |
2 |
|a Catalysis.
|0 (DNLM)D002384
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| 650 |
2 |
2 |
|a Flavoproteins
|x chemistry.
|0 (DNLM)D005420Q000737
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| 650 |
|
7 |
|a SCIENCE
|x Life Sciences
|x Biology.
|2 bisacsh
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| 650 |
|
7 |
|a Flavins.
|2 fast
|0 (OCoLC)fst00927097
|
| 650 |
|
7 |
|a Flavoproteins.
|2 fast
|0 (OCoLC)fst00927107
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| 655 |
|
4 |
|a Electronic books.
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| 830 |
|
0 |
|a Methods in enzymology ;
|v v. 620.
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| 856 |
4 |
0 |
|3 ScienceDirect
|u https://www.sciencedirect.com/science/bookseries/00766879/620
|z Full Text via HEAL-Link
|
