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04623nam a2200505 4500 |
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on1114334858 |
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20200326101203.0 |
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m o d |
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cr cnu---unuuu |
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190829s2019 mau o 000 0 eng d |
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|a OPELS
|b eng
|e rda
|e pn
|c OPELS
|d EBLCP
|d OCLCF
|d UKAHL
|d OCLCQ
|d CASUM
|d OCLCO
|d YDXIT
|d SFB
|d GrThAP
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|a 1142630173
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|a 9780128183595
|q (print)
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|a 9780128183601
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|a 0128183608
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|a 0128183594
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|a (OCoLC)1114334858
|z (OCoLC)1142630173
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|a QP624
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|a 572.8/6
|2 23
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|a DNA sensors and inflammasomes /
|c edited by Jungsan Sohn.
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|a Cambridge, MA :
|b Academic Press,
|c 2019.
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| 300 |
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|a 1 online resource
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|a text
|b txt
|2 rdacontent
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|a computer
|b c
|2 rdamedia
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|a online resource
|b cr
|2 rdacarrier
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1 |
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|a Methods in enzymology ;
|v volume 625
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|a Title details screen.
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|a Front Cover; DNA Sensors and Inflammasomes; Copyright; Contents; Contributors; Preface; Chapter One: Intercellular communication in the innate immune system through the cGAS-STING pathway; 1. Introduction; 2. cGAS-STING signaling; 2.1. Inter-cellular communication; 2.2. Type 1 IFN; 3. Immuno-signaling to neighboring cells through gap junctions; 3.1. cGAMP in virus particles; 3.2. Extracellular vesicles; 4. Exosomes and immune responses to virus infections; 5. Exosomes and immune responses to bacterial infections; 6. Concluding remarks; References
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|a Chapter Two: Analysis of human cGAS activity and structure1. Introduction; 2. A genetic assay for screening cGAS activity using bacteria; 2.1. Assay setup and considerations; 2.2. Protocol; 2.3. Future applications; 3. Crystallization and structural determination of the human cGAS-DNA complex; 3.1. Preparation of human cGAS protein and DNA; 3.1.1. Equipment; 3.1.2. Reagents; 3.1.3. Protocol; 3.2. Assays to assess human cGAS-DNA complex formation; 3.2.1. Equipment; 3.2.2. Reagents; 3.2.3. Protocol; 3.3. Human cGAS-DNA crystallization protocol; 3.3.1. Equipment; 3.3.2. Reagents; 3.3.3. Protocol
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|a 3.4. Human cGAS-DNA structure determination3.4.1. Equipment; 3.4.2. Reagents; 3.4.3. Protocol; 4. Crystallization of a human cGAS-DNA-ligand complex; 4.1. Crystal soaking and human cGAS-DNA-ligand crystallization; 4.2. Protocol for ligand soaking and crystal harvest; 4.2.1. Reagents; 4.2.2. Protocol; 5. Summary; Acknowledgments; References; Chapter Three: Chemical synthesis, purification, and characterization of 3-5-linked canonical cyclic dinucleotides (CDNs); 1. Introduction; 2. Synthesis of crude CDNs; 2.1. Chemicals; 2.2. Equipment; 2.3. Synthesis procedure for crude c-AMP-CMP
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|a 3. Purification of crude CDNs3.1. Preparation of HPLC buffers; 3.2. Purification protocol for crude c-AMP-CMP; 3.3. HPLC purification of crude c-AMP-CMP; 4. Characterization of CDNs; 4.1. Materials and equipment; 4.2. MS analysis of c-AMP-CMP fractions; 4.3. NMR analysis of c-AMP-CMP; 5. Summary and prospects; Acknowledgments; References; Chapter Four: An SPR-based analysis of cGAS substrate KD and steady-state KM values; 1. Introduction; 2. Consideration for the immobilization of proteins, multimers, and the cGASds-DNA complex; 3. Considerations for SPR-based enzymology; 4. Protocols
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|a 4.1. Protein reagents4.2. Chemical reagents; 4.3. Equipment; 4.4. Chip preparation and protein capture; 4.5. cGAS-ligand binding studies; 4.5.1. Preparation of samples; 4.5.2. Details of injections and data collection; 4.5.3. Data analysis; 4.6. cGAS activity assays; 4.6.1. Preparation of samples; 4.6.2. Details of injections and data collection; 4.6.3. Data analysis; 5. Summary; References; Chapter Five: A pyrophosphatase-coupled assay to monitor the NTase activity of cGAS; 1. Introduction; 2. Materials; 2.1. Chemicals, materials and enzymes; 2.2. Buffers and assay reagents
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| 504 |
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|a Includes bibliographical references.
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| 650 |
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|a DNA.
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1 |
2 |
|a DNA
|x chemistry.
|0 (DNLM)D004247Q000737
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| 650 |
2 |
2 |
|a Biosensing Techniques.
|0 (DNLM)D015374
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| 650 |
2 |
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|a CARD Signaling Adaptor Proteins.
|0 (DNLM)D053468
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| 650 |
2 |
2 |
|a Inflammasomes.
|0 (DNLM)D058847
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| 650 |
2 |
2 |
|a Signal Transduction.
|0 (DNLM)D015398
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| 650 |
|
7 |
|a DNA.
|2 fast
|0 (OCoLC)fst00886555
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| 655 |
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4 |
|a Electronic books.
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| 700 |
1 |
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|a Sohn, Jungsan,
|d 1978-
|e editor.
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| 830 |
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|a Methods in enzymology ;
|v v. 625.
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| 856 |
4 |
0 |
|3 ScienceDirect
|u https://www.sciencedirect.com/science/bookseries/00766879/625
|z Full Text via HEAL-Link
|
