Immunocytochemistry A Practical Guide for Biomedical Research /

• Main Author: Burry, Richard W. (Author)
• Corporate Author: SpringerLink (Online service)
• Format: Electronic eBook
• Language: English
• Published: New York, NY : Springer New York, 2010.
• Subjects: Life sciences.; Immunology.; Biochemistry.; Cell biology.; Microscopy.; Life Sciences.; Biochemistry, general.; Life Sciences, general.; Animal Biochemistry.; Biological Microscopy.; Cell Biology.

Immunocytochemistry is a microscopic method that uses antibodies to localize proteins in tissue sections and cell cultures. Dr. Burry’s book is the first practical guide to planning, performing, and evaluating immunocytochemical experiments designed for novice immunocytochemists. This book’s predomi...

Table of Contents:

• Antibodies
• Sample Preparation/Fixation
• Tissue Sectioning
• Blocking and Permeability
• Labels for Antibodies
• Application Methods
• Controls
• Method and Label Decision
• Single Antibody Procedure
• Multiple Antibodies Different Species
• Multiple Antibodies from the Same Species
• Fluorescent Microscopy and Imaging
• Troubleshooting
• Electron Microscopic Immunocytochemistry.